Protein Detection

Protein ladder sets, Western blot detection buffers, reagent and substrate series

CLEARLY Protein Ladder Molecular Weight Markers

Convenient and ready-to-use Ladder sets
Mixture of 11 highly purified recombinant proteins
Load directly on gels - no pre-heating is necessary
CLEARLY Protein Ladder (Unstained) is recommended as a precise molecular weight standard.
Easily monitor the efficiency of protein transfer to a western blot

TaKaRa CBB Protein Safe Stain

Highly sensitive Coomassie Brilliant Blue G-250-based stain detects as little as 8 ng of protein
Visualize protein bands in approximately 5 min, with maximum sensitivity after 60 min
Safe handling—does not contain methanol or acetic acid; destain gels with deionized water
This product can be used to stain SDS-PAGE or native PAGE gels quickly

Western Blot Blocking Buffer

Suppresses non-specific signals is critical to obtaining clear, specific Western blot results. TaKaRa Bio offers several specialized, ready-to-use blocking buffers.

1) Western BLoT Blocking Buffer (Protein Free)

completely chemically defined and free of protein components
Provides higher sensitivity than other commonly used blocking agents (nonfat milk)
Excellent for detection of phosphorylated proteins

2) The Western BLoT Blocking Buffer (Fish Gelatin)

Blocking reagent of fish origin
It is less likely to cross-react with mammalian antibodies than conventional blocking reagents, such as nonfat milk or BSA

Western BLoT Chemiluminescent Substrate Series

Chemiluminescent substrates for detecting horseradish peroxidase (HRP)-labeled antibodies used during Western blotting.

Features

Product name	Features	Detection Limit	Detection Method	Membrane
Western BLoT Chemiluminescence HRP Substrate	Stronger signal than other equivalent ECL substrates.	Picogram	X-ray film	Nitrocellulose, PVDF
Western BLoT Quant HRP Substrate	Long-lasting, stable signal. High sensitivity and quantitative accuracy.	Low picogram	CCD camera, X-ray film	Nitrocellulose, PVDF
Western BLoT Hyper HRP Substrate	Quantitative detection even for low-abundance proteins. Strong signal when high dilution factor for the primary antibody is used.	Femtogram	CCD camera, X-ray film	Nitrocellulose, PVDF
Western BLoT Ultra Sensitive HRP Substrate	Highest sensitivity in the series.	Low femtogram	CCD camera, X-ray film	Nitrocellulose, PVD

Western BLoT Immuno Booster

Highly sensitive detection of low abundance proteins without having to change HRP substrate or the amount of antibody
Protein-free version is available
For variety of immunoassays, including Western blotting and ELISA
Enhance antigen-antibody interactions and thereby increase final detection sensitivity

Western BLoT Rapid Detect v2.0

Rapid detection of primary antibodies using an antibody-free IgG Detector Solution (HRP-labeled).
Simultaneous detection of multiple primary antibodies
Detection of minute quantities of antigen that cannot be detected using conventional techniques with a labeled secondary antibody

Western Blot Stripping Buffer

Efficient antibody removal from Western blot membranes with little loss of bound protein
Fast, simple protocol (immerse blot in Stripping Buffer and incubate at room temperature for 30 minutes)