High fidelity PCR polymerases
Polymerases that provide maximum fidelity and longer product lengths
1. PrimeSTAR HS polymerase
PrimeSTAR HS polymerases provide high accuracy during amplification of large DNA products with an extremely low error rate of only 12 errors / 250 kb. The antibody-mediated hot-start function helps reduce background and improve specificity. PrimeSTAR HS polymerase also has a high tolerance to different reaction conditions. It is suitable for various applications such as high-precision PCR, amplification of cDNA libraries, cDNA cloning for protein expression or site-controlled mutagenesis and mutant genotyping (e.g. SNP analysis).
Main features:
- High accuracy and strong exonuclease activity
- Ultra-low error rate, only 12 errors per 250kb
- Suitable for GC-rich templates amplification
- High tolerance to different reaction conditions (a single PCR cycle protocol can be used to amplify products of different sizes)
- Target amplification up to 8.5 kb (human genomic DNA), 10 kb (E. coli genomic DNA) and 22 kb (lambda DNA)
- Hot-start PCR enzyme
STANDARD VERSIONS:
PrimeSTAR® HS DNA Polymerase
- Supplied as polymerase, optimized buffer (Mg2+ plus) and dNTP mixture in separate tubes
PrimeSTAR® HS DNA polymerase (premix)
- 2X hot-start PCR master mix with PrimeSTAR buffer and dNTPs
OTHER VERSIONS
PrimeSTAR HS WITH GC buffer
- Hi-Fi amplification for the heaviest GC-rich content (75% or greater).
- Read more: GC-rich PCR polymerases
PrimeSTAR GXL Products
- Hi-Fi and the ability to amplify long and GC- or AT-rich targets
- Read more: GC-rich PCR polymerases
2. PrimeSTAR Max DNA polymerase
PrimeSTAR Max DNA polymerase is a combination of PrimeSTAR HS polymerase and elongation factor. Standardization of the extension step time makes all versions of PrimeSTAR Max DNA Polymerase suitable for reactions with excessive nucleic acids, which are difficult to amplify due to non-specific bonding. PrimeSTAR Max DNA polymerase Ver. 2 is the polymerase with the highest fidelity and fastest extension rate among Takara Bio enzymes. The processing speed is ~5 sec/kb.
Main features:
- Modified PrimeSTAR polymerase in combination with elongation factor
- Template length: up to 30 kb with human gDNA or 13.5 kb with cDNA
- Excellent performance on templates and targets rich in GC or AT with repeating elements
- Also for samples containing excess nucleic acids
- For a wide range of template volumes
- Antibody-mediated hot-start formulation
STANDARD VERSIONS
PrimeSTAR® Max DNA polymerase
- 2X hot-start PCR master mix in premix containing Mg2+
PrimeSTAR® Max DNA polymerase Ver.2
- 2X premixed reagent with antibody-mediated hot start formula
3. Advantage HD DNA polymerase
Advantage HD DNA polymerase with maximum fidelity is suitable for applications where accuracy is key (cDNA cloning, site-controlled mutagenesis and mutational genotyping). The enzyme has 3' → 5' exonuclease activity and an extremely low error rate. It also performs well with samples with high GC pair content and complex structure.
Main features:
- Provides excellent accuracy and efficiency
- Low error rate: Only 12 errors per 250,000 basis points
- Generates error-free PCR products from targets of any complexity up to 8.5kb
- Performs well on GC-rich templates
- The hot-start antibody is integrated
- For critical applications such as gene cloning and amplification of cDNA for library building, infusion cloning, etc.
STANDARD VERSIONS
Advantage®: HD polymerase blend
- In the form of polymerase and 5X Advantage HD buffer (contains MgCl2), dNTPs must be purchased separately
4. CloneAmp HiFi PCR Premix
The CloneAmp HiFi PCR Premix is specially designed for use with the In-Fusion cloning system. This premix is exceptionally accurate and efficient in DNA synthesis. It is suitable for high-throughput applications and mounting multiple cloning samples.
Main features:
- Provides exceptionally accurate and efficient DNA amplification
- Low error rate: 12 mismatched bases per 542,580 bases
- Fast priming and lengthening (5 sec/kb) enable fast PCR reactions
- Hot-Start antibody prevents nonspecific amplification
- Works with long (up to 10kb) and GC-rich templates
STANDARD VERSIONS:
CloneAmp™ HiFi PCR Premix
- 2X PCR already premixed master mix, includes CloneAmp HiFi polymerase, dNTPs and optimized buffer
Documents and resources
For more information about PrimeSTAR HS polymerase, visit the manufacturer’s website.
For more information about PrimeSTAR Max DNA polymerase, visit the manufacturer’s website.
For more information about Advantage HD DNA polymerase, visit the manufacturer’s website.
For more information about CloneAmp™ HiFi PCR Premix, visit the producer’s website.
