qPCR Reagents

Broad range of reagents suitable for quantitative PCR. Choose from intercalation dye-based kits with our TB Green or from kits dedicated to probe detection. Premixed formats of the kits allow user to simply add DNA sample, primers and probes and start the reaction.

1. DYE-BASED DETECTION

TB Green Premix Ex Taq II FAST qPCR

Kit contains mutant Taq DNA polymerase – Ex Taq, which is suitable for high-speed PCR reactions. In the mix the TB Green intercalating dye is added for Real time PCR applications. To reach higher inhibitor stability, Tli RNaseH and UNG are part of the mix.

Main Features:

• Ultimate specificity—suppressed non-specific amplification
• High-speed reaction
• Contains TB Green intercalating dye

STANDARD VERSIONS:

TB Green® Premix Ex Taq™ II FAST qPCR

• Consists of TB Green Ex Taq II FAST premix and EASY Dilution II (diluent for qPCR sample)

TB Green Advantage qPCR premixes

2X concentrated mastermix in premix format containig full length Taq polymerase, hot-start antibody and TB Green.

Main Features:

• Contains TB Green intercalating dye
• Optimized for use with the laser-, lamp-, or LED-based real-time instrument of your choice
• Compatible with all real-time PCR instruments

STANDARD VERSIONS:

TB Green® Advantage® qPCR Premix

• 2X master mix including TB Green dye, hot-start antibody, dNTPs, and buffer and two tubes with ROX passive reference dye

TB Green Premix Ex Taq (Tli RNase H Plus)

TB Green Premix Ex Tay (Tli RNase H Plus) is suitable for most real-time PCR applications such as gene expression analyses or genotyping. Also, 2X Takara Ex Taq HS enzyme premix contains Tli RNase H, a heat-resistant RNase H for qPCR inhibition minimization. With this product conduct fast qPCR reactions are possible.

Main Features:

• RNase H included in reaction mix: reduces inhibition from mRNA/cDNA hybrids
• Eliminates need for RNase H digestion step: when using low RNase H RTs
• Amplification of longer fragments (up to 570 kb)
• High sensitivity: detects as few as 100 copies
• Excellent standard curves for various real time instruments have been established
• Compatible with all qPCR instruments

STANDARD VERSIONS:

TB Green® Premix Ex Taq™ (Tli RNase H Plus)

• Premix format supplied together with two separate tubes of ROX passive reference dye

TB Green® Premix Ex Taq™ (Tli RNase H Plus), 1 x 5 mL

• Bulk version, Premix format supplied together with separate tube of ROX passive reference dye

TB Green® Premix Ex Taq™ (Tli RNase H Plus), 1 x 5 mL, ROX Plus

• Bulk version, Premix format, ROX passive reference dye included inside the mix

TB Green® Premix Ex Taq™ (Tli RNase H Plus), 5 x 5 mL, ROX Plus

• Bulk version, Premix format, ROX passive reference dye included inside the mix

TB Green Premix DimerEraser (Perfect Real Time)

Specifically designed premix for Real-Time PCR method using TB Green intercalating dye. Thanks to the improved buffer system this product has improved reaction specificity compared to the TB Green Premix Ex Taq II (Perfect Real Time). Works with broad range of sample concentrations. This product suppresses the formation of primer dimers, making accurate, quantitative analysis possible.

Main Features:

• Quick and accurate detection and quantification of target
• Easy-to-use 2X premix reagent including TB Green
• High amplification efficiency and highly sensitive detection
• Buffer system is optimized for real-time PCR
• Accessory protein suppresses nonspecific amplification and primer-dimer amplification

STANDARD VERSIONS:

TB Green® Premix DimerEraser™ (Perfect Real Time)

• Provided as a 2X premix, supplied with ROX dye in two separate tubes

Terra qPCR Direct TB Green Premix

 2X master mix that lets you perform real-time PCR (qPCR) directly on crude extracts or dirty samples (such as mouse tail and ear biopsies, or leaf cuttings). Terra PCR Direct polymerase works with targets with high GC-pair content (up to 70 %) and with length of up to 2 kb.

Main Features:

• Perform real-time PCR with crude tissue extracts or dirty samples
• Easily amplify GC-rich targets
• Convenient 2X master mix contains TB Green dye

STANDARD VERSIONS:

Terra™ qPCR Direct TB Green® Premix

• Kit contains polymerase mix and positive control (with proprietary primers), specific primers need to be added, ROX dye supplied in separate tubes

TB Green Premix Ex Taq II (Tli RNase H Plus)

Premix suitable for amplification, where high sensitivity is needed. It suppresses primer dimer amplification. Tli RNase H Plus degrades residual mRNA in the input cDNA and therefor lowers the qPCR inhibition. Premix can be used for amplification of targets with high GC-pair content.

Main Features:

• Optimized for real time dye-based qPCR detection
• Excels with GC-rich targets
• 2X master mix provides convenience
• High sensitivity: detects fewer than 100 copies
• Minimization of PCR inhibition - Tli RNAse H
• Optimized buffer and anti-Taq antibody mediated HS polymerase
• Compatible with all qPCR instruments

STANDARD VERSIONS:

TB Green® Premix Ex Taq™ II (Tli RNase H Plus)

• Premix format supplied together with two separate tubes of ROX passive reference dye

TB Green® Premix Ex Taq™ II (Tli RNase H Plus), 1 x 5 mL

• Bulk version, Premix format supplied together with separate tube of ROX passive reference dye

TB Green® Premix Ex Taq™ II (Tli RNase H Plus), 1 x 5 mL, ROX Plus

• Bulk version, Premix format, ROX passive reference dye included inside the mix

TB Green® Premix Ex Taq™ II (Tli RNase H Plus), 5 x 5 mL, ROX Plus

• Bulk version, Premix format, ROX passive reference dye included inside the mix

2. PROBE-BASED DETECTION

Premix Ex Taq (Probe qPCR)

Premix Ex Taq (Probe qPCR) is suitable for high-speed PCR detection over a broad dynamic range. Also, 2X Takara Ex Taq HS enzyme premix contains Tli RNase H, a heat-resistant RNase H for qPCR inhibition minimization. With this product conduct fast qPCR reactions are possible. Can be used for variety of applications such as gene expression studies.

Main features:

• For real time PCR and TaqMan probe detection
• Contains optimized buffer and Ex Taq HS polymerase

• Includes heat-resistant Tli RNase H for mRNA inhibition minimisation
• Compatible with all qPCR instruments
• Convenient 4 °C storage possible

STANDARD VERSIONS:

Premix Ex Taq™ (Probe qPCR)

•  2X premix includes Takara Ex Taq HS and buffer, two ROX dyes supplied in separate tubes

Premix Ex Taq™ (Probe qPCR), ROX Plus

• 2X premix includes Takara Ex Taq HS and buffer, ROX dye included in the premix

Premix Ex Taq (Perfect Real Time)

Specifically designed for qPCR reactions with either probes or intercalating dyes. With this reagent, real-time PCR results with high sensitivity, wide dynamic range, and accurate quantification can be obtained.

Main Features:

• High specificity: includes optimized buffer and Ex Taq HS for multiplex
• High sensitivity: detects as few as 10 target copies
• Wide dynamic range: > 6 log
• Accurate quantitation: produces excellent standard curves with numerous real time instruments
• Compatible with all qPCR instruments

STANDARD VERSIONS:

Premix Ex Taq™ DNA Polymerase (Perfect Real Time)

• 2X premix includes Takara Ex Taq HS and buffer, two ROX dyes supplied in separate tubes

Premix Ex Taq™ DNA Polymerase (Perfect Real Time), 25 mL

• 25 ml of 2X premix includes Takara Ex Taq HS and buffer, two ROX dyes supplied in separate tubes