CHIP-Seq Protein-DNA Interaction

Chromatin immunoprecipitation sequencing (ChIP-seq) has facilitated tremendous progress for epigenetics research. Given that many proteins of interest (e.g. transcription factors and modified histones) are associated with a limited number of specific genomic loci, the amount of DNA recovered by chromatin immunoprecipitation can be quite low, posing challenges for the generation of representative ChIP-seq libraries.

Main features:

• Reverse cross-link, elute, and purify DNA captured by chromatin immunoprecipitation (ChIP)
• Ligation-free protocol
• Generates libraries in less than 4 hours
• Based on SMART technology
• No need for enzyme cleanup

AVAILABLE KITS: 

DNA SMART ChIP-Seq Kit

• Inputs of 100 pg – 10 ng of single or double-stranded DNA
• Highly sensitive kit generates robust sequencing libraries from low-input DNA samples
• Illumina-ready sequencing libraries
• Multiplexing of up to 48 samples

ChIP Elute Kit

• Cross-link reversal and elution of ChIP DNA
• Replaces long and tedious protocols for the final stages of ChIP experiments
• ssDNA can be recovered in one hour compared to traditional methods that may require overnight incubation
• Directly compatible with the DNA SMART ChIP-Seq Kit