CHIP-Seq Protein-DNA Interaction

Protein-DNA interaction analysis of a genome using chromatin immunoprecipitation sequencing

Chromatin immunoprecipitation sequencing (ChIP-seq) has facilitated tremendous progress for epigenetics research. Given that many proteins of interest (e.g. transcription factors and modified histones) are associated with a limited number of specific genomic loci, the amount of DNA recovered by chromatin immunoprecipitation can be quite low, posing challenges for the generation of representative ChIP-seq libraries.

Main features:

Reverse cross-link, elute, and purify DNA captured by chromatin immunoprecipitation (ChIP)
Ligation-free protocol
Generates libraries in less than 4 hours
Based on SMART technology
No need for enzyme cleanup

AVAILABLE KITS:

DNA SMART ChIP-Seq Kit

Inputs of 100 pg – 10 ng of single or double-stranded DNA
Highly sensitive kit generates robust sequencing libraries from low-input DNA samples
Illumina-ready sequencing libraries
Multiplexing of up to 48 samples

ChIP Elute Kit

Cross-link reversal and elution of ChIP DNA
Replaces long and tedious protocols for the final stages of ChIP experiments
ssDNA can be recovered in one hour compared to traditional methods that may require overnight incubation
Directly compatible with the DNA SMART ChIP-Seq Kit