Takara Bio
Long Read mRNA-Seq
Long Read sequencing libraries from low input RNA samples
SMART-Seq mRNA Long Read kit enables synthesis of Oxford Nanopore (ONT) sequencing libraries with average read lengths (N50) of ~2 kb and detection of full-length transcripts with over 8 kb. cDNA synthesis is powered by SMART (Switching Mechanism at 5' End of RNA Template) technology, which enables analysis of transcript isoforms, gene fusions, point mutations, etc. from low sample inputs.
Main features:
- Input: 10 pg–100 ng total RNA or 1–1,000 intact cells
- Read lengths (N50) of ~2 kb, detecting full-length transcripts over 8 kb
- Single tube protocol
- Accurate representation of GC-rich transcripts
- Multiplexing of up to 96 samples
- Workflow completed in 1.5 days
- Libraries compatible with Oxford Nanopore Technologies (ONT) platform
Documents and resources
For more information visit producer’s website.
