Takara Bio mRNA-Seq kits are based on the SMART (Switching Mechanism at 5' End of RNA Template) technology for cDNA synthesis, which enable capturing the entire sequence of the mRNA transcript. Kits provide high-quality RNA-seq data from low input material amounts and are robust and easy to use. Obtained sequencing libraries are compatible with Illumina platforms. Takara Bio also provides user-friendly bioinformatics software Cogent for rapid data analysis and visualization.
Main features:
- Provide full length transcript information
- Single-tube protocol for minimal sample loss
- Works with ultra-low and single-cell inputs
- Full length oligo(dT) priming
- With compatible UDIs can be multiplexed up to 384 samples
- Libraries compatible with Illumina sequencing platforms
AVAILABLE PRODUCTS:
SMART-Seq® mRNA LP (with UMIs)
- Input range: 10 pg–100 ng total RNA (RIN ≥ 8), or 1–1,000 cells
- Incorporates UMIs for higher accuracy
- Includes cDNA synthesis and library preparation chemistry
- UDIs sold separately
SMART-Seq® mRNA LP
- Input range: 10 pg–100 ng total RNA (RIN ≥ 8), or 1–1,000 cells
- Designed for cDNA synthesis and library preparation
- Identify the highest number of genes; detect gene fusions, isoforms, SNPs
- UDIs sold separately
SMART-Seq® mRNA
- Input range: 10 pg–100 ng total RNA (RIN ≥ 8), or 1–1,000 cells
- Designed for cDNA synthesis
SMART-Seq® mRNA Single Cell LP
- Input range: 1 cell
- Robust, full-length chemistry
- Designed for cDNA synthesis and library preparation
- Works well even with cells with very low RNA content and nuclei
- Automation friendly
- UDIs sold separately
SMART-Seq® mRNA HT LP
- Input range: 1–100 cells or 10 pg–1 ng of total RNA
- Easy to automate workflow
- Capture full-length transcript coverage, a low percentage of rRNA reads, and a broad representation of GC-rich transcripts
- Designed for cDNA synthesis and library preparation
- UDIs sold separately
SMART-Seq® mRNA HT
- Input range: 1–100 cells or 10 pg–1 ng of total RNA
- Designed for cDNA synthesis
