Single-Cell Whole Genome Amplification

Takara Bio kits for single-cell DNA-Seq library preparation are powered by PicoPLEX technology, which enables whole-genome amplification (WGA) with unsurpassed reproducibility and coverage uniformity. With low starting material amounts PicoPLEX based kits provide reliable results from challenging samples such as formalin-fixed single cells. PicoPLEX technology can also be used as standalone for non-NGS applications.

Main features:

• Optimized for detection of copy number variants (CNVs), single nucleotide variants (SNVs), indels, and small structural variants
• Four step protocol
• Library prepared in 3 hours with less than 30 minutes hands-on time
• UDIs set allows multiplexing of up to 384 samples

AVAILABLE KITS: 

PicoPLEX® Gold Single Cell DNA-seq Kit

• Single-cell DNA library preparation kit
• Low allele drop-in and drop-out rates
• Input material: 1–5 mammalian cells or equivalent gDNA amount
• UDIs sold separately

PicoPLEX® WGA Kit

• Input material: 1-10 human cells or equivalent gDNA amount
• Standalone WGA
• Designed for copy number variations (CNVs) detection, including preimplantation genetic testing for aneuploidy (PGT-A)

PicoPLEX Single Cell WGA Kit

• Input material: 1-10 human cells or equivalent gDNA amount
• Standalone WGA
• Improved evenness and reproducibility of amplification and higher yields compared to PicoPLEX® WGA Kit

PicoPLEX® Single Cell WGA Kit v3

• Input material: 1-10 human cells or equivalent gDNA amount
• Standalone WGA
• Improved formulation, additional refinements to allow for more accurate calling of single-nucleotide variants (SNVs)