Seeker Spatial Transcriptomics Kit

With Seeker whole transcriptome, spatial mapping of fresh frozen tissues is possible with 10 μm resolution. Slices of the tissue are placed on a Seeker pile, where tightly-packed, spatially-indexed beads are to index the tissue and ensure the spatial information preservation. Polyadenylated mRNA is captured and hybridized on the spatially-indexed beads before reverse transcription. Bead dissociation and tissue digestion is follower by cDNA synthesis and NGS library preparation. Sequencing data is later analyzed with The Seeker bioinformatics pipeline and spatial gene expression maps can be generated.

Main features:

• Input: fresh frozen tissue section
• Offered in 3 mm x 3 mm or 10 mm x 10 mm tile sizes without sacrificing resolution
• 10 μm resolution
• Compatible with eucaryotic organism
• 8-hour workflow with no need of specialized devices or microscopy
• Analyze any polyadenylated RNA 
• Need to be paired with Nextera® XT DNA Sample Preparation Kit to generate Illumina compatible library

AVAILABLE FORMATS:

Seeker™ 3x3 Bundle V1.1

• 3X3 mm tile
• Includes RNA hybridization, cDNA synthesis, and cDNA amplification
• Amount sufficient for barcoding of 8 slides
• Seeker™ Dual Indexing Primer Kit V2 required to be purchased

Seeker™ 10x10 Bundle V1.1

• 10X10 mm tile
• Includes RNA hybridization, cDNA synthesis, and cDNA amplification
• Amount sufficient for barcoding of 8 slides
• Seeker™ Dual Indexing Primer Kit V2 and Seeker™ Reaction Chamber Adapter purchase required

Seeker™ Dual Indexing Primer Kit V2

• Dual indexing primers required for the library preparation
• Amount for up to 40 3 mm x 3 mm Seeker tiles or five 10 mm x 10 mm Seeker tiles

Seeker™ Reaction Chamber Adapter

• Allows processing 10 mm x10 mm Seeker tiles in thermal cycler
• Need to be purchased for 10 mm x10 mm Seeker tile workflow