Cloning Vectors

E. coli-yeast shuttle vectors

• pAUR vector series (pAUR101 DNA, pAUR112 DNA, pAUR123 DNA, pAUR1135 DNA, pAUR224 DNA, pAUR316 DNA)
• Constructed for a particular application such as marker removal or chromosomal integration
• For: Saccharomyces cerevisiae, Schizosaccharomyces, Saccharomyces pombe or Aspergillus nidulan

In-Fusion REady Fluorescent Protein Vectors

• Prelinearized version of popular DsRed-Monomer-N1/C1 and AcGFP1-N1/C1 for fast and efficient PCR cloning using In-Fusion 
• One step and flexible reaction allowing parallel cloning of fusion proteins with different colours at either N- or C- terminus

 

Chloramphenicol Resistant pUC Vectors

• Vectors pHSG396 and pHSG398 contain the chloramphenicol resistance gene
• Suitable for lac promoter enabled target gene cloning and expression
• Suitable for DNA sequencing using an M13 primer or Long DNA sequencing

 

DNA Cloning Vectors

• Can be used for wide range of cloning reactions
• See the vectors:
  • pKF 18k-2/19k-2 DNA
  • pSTV 28/29 DNA
  • pTV118N DNA
  • pTWV228 DNA
  • pUC118 DNA

Kanamycin Resistant pUC Vectors

• Vectors pHSG298 and pHSG299
• Contain origin of replication (ori), and the beta-galactosidase coding gene lacZ
• Enable high target gene expression

 

pUC118 and pUC119 Vectors

• Bacteriophage packaging of vectors contains an intergenic region of the M13 phage DNA,
• Enabling their replication in the form of single stranded DNA once transformed into bacteria with the helper phage M13K07

 

pUC18 and pUC19 Plasmids

• Enable successful cloning of larger DNA fragments than the M13 mp18 RF Phage Vector

 

T-Vectors pMD20 and pMD19 for TA Cloning

• T-Vectors are linearized PCR cloning vectors (EcoRV cleaved)
• Have single 3’-terminal thymidine residues (dT) at both ends
• T-overhangs at the cloning site improve the efficiency of ligation of PCR products that contain dA-overhangs

 

Pyrithiamine Vectors

• Contain an ampicillin resistance gene as the selection marker for E.coli and pyrithiamine resistance gene as the selection marker for Aspergillus

 

Aureobasidin A Vectors

• The pAUR vector series include six E. coli yeast shuttle vectors
• Each constructed for a particular application in either:
  • Saccharomyces cercevisiae
  • Schizosaccharomyces
  • Saccharomyces pombe
  • Aspergillis nidulans
• Include a novel drug resistance selective marker that confers:
  • Aureobasidin A resistance in transformed yeast
  • Filamentous fungal species