AVV Transduction

1) AAV PACKAGING

293T Cell Line

• Suitable for adeno-associated virus (AAV) packaging
• Supports the high level expression of viral proteins in high titers

AAV Helper-Free Packaging Plasmid Set

• Two plasmids (pRC-mi342 and pHelper) designed to get a high titer AAV2
• AAV2 particles are generated without the use of a helper virus

2) AAV PURIFICATION AND TITRATION

AAVpro Extraction Solution

• Is an extraction buffer that increase AAV yield at least 3-fold
• It provides a simple centrifugation method
• Can be used for any AAV serotype

AAVpro Concentrator

• Suitable for any AAV serotype
• Without need of ultracentrifugation
• Incorporates purification a filtration steps
• Process up to 150 ml of sample

AAVpro Titration Kit

• Is a qPCR titration kit
• Contains all the components necessary for extraction of AAV particles and qPCR detection
• The workflow lasts approximately 2,5 hours

AAVpro Purification Kits

• Column based purification system
• Without need for ultracentrifugation
• Reduction of freeze-thaw steps
• Available in broad range of versions (AAV2 or All serotype compatibility)

3) AAV VECTOR SYSTEMS

AAVpro Helper Free System (AAV2)

• Kit for the preparation of AAV2
• No need of helper virus
• Includes three plasmids encoding the factors necessary to create AAV particles
• Extraction reagents included

pAAV ZsGreen1 Vector

• Can be used as a positive control to AAVpro Helper Free System (AAV2)
• Expression of the green fluorescent protein ZsGreen with exeptionally high brightness

AAV Tet-Inducible Expression System

• All the components for tetracycline inducible expression on a single vector
• Tet-On 3G transactivator is expressed in from the human phosphoglycerate kinase 1 (PGK) promoter
• Cloned gene of interest is expressed from the P_TRE3GS promoter
• This all in one design has been shown to result in up to 25 000 fold induction

AAV Cre Recombinase System

• Kit for preparation of AAV2 particles to deliver a Cre recombinase gene under the control of the CMV promoter
• It can be used in transduction of target cells containing a floxed DNA sequence with the AAV2-CRE Recombinase particles
• Results in recombination between the loxP sites or it can be used for generating the transgenic mice

AAV2-CRISPR/Cas9 System

• Viral delivery of the Cas9/sgRNA complex allows to edit hard to transfect cells
• The Cas9 gene is splitted between two plasmids with 1,6 kb region of homology which produce the full length Cas9 gene
• Expression of a human microRNA miR-342 from the packaging plasmid significantly increases AAV titer

AAV-LacZ Particles

• Kit to prepare AAV2 particles for delivery of the lacZ reporter gene to mammalian cells under the control of the CMV promoter
• Can be prepared without a helper virus
• The lacZ gene encodes the commonly used reporter, enzyme beta-galactosidase