Inducible Protein Stabilization

The technology is based on a destabilizing domain that can confer its instability to a fused protein of interest. This destabilization can be rescued in a reversible and dose-dependent manner with the addition of a small molecule specific to the destabilizing domain, Shield1. The destabilizing domain is mutant L106P of FKBP12 (12 kD), which is rapidly degraded in mammalian cells. The FKBP12 protein itself is well-characterized for its ability to form a tight complex with immunosuppressive drugs.

1) PLASMID SYSTEMS

Protein Regulation with Rapid Kinetics-ProteoTuner Systems rapidly regulate the quantity of your protein of interest, at the post translational level. The ProteoTuner systems use a powerful, unique technology to control your protein in living cells.

Main features:

• Rapid kinetics: Protein level changes in minutes allows accurate functional analysis
• Precise tuning: Precise control of protein level is key to analyzing biological activity
• Reversible control: 'Protein on' to 'off' and back makes multiple studies easier

AVAILABLE PRODUCTS:

Shield1 Ligand

• Membrane permeable small molecule
• Binds specifically to the DD degron, to inactivate protein degradation
• No toxic effect on cells
• Can be used in cells and in animal models

2) PROTEOTURNER ANTIBODY

The Destabilization Domain Monoclonal Antibody recognizes the destabilization domain (destabilization domain-tag) in all ProteoTuner Shield Systems (N and C versions).

Main features:

• Western blot and immunocytochemistry detection of destabilization domain tagged proteins
• Applicable to ProteoTuner Shield Systems (both and N and C versions)

3) VIRAL SYSTEMS

ProteoTuner Lentivirus and Retrovirus Systems analyzing protein function is a key focus in cell biology research. Systems make it possible to investigate the function of a specific protein of interest directly by rapidly changing the abundance of the protein itself.