His-Tagged Protein Purification

We offer broad range of solutions for His-tagged protein purification. For fast, easy purification under a wide range of conditions. To achieve exceptional purity, use TALON cobalt-based IMAC resin. Takara Bio also provides high capacity His60 Ni-IDA and Magnetic beads.

1) CAPTUREM TECHNOLOGY

Capturem product line with nickel-functionalized membranes for his-tagged purification are fast solution with only 5-30 minutes from loading a sample through elution and are designed for recombinant protein purification. Products, which range from spin columns to filtration devices, are suitable for work with both mammalian and bacterial cells in room temperature.

AVAILABLE PRODUCTS:

The Capturem His-Tagged Purification Miniprep Kit

• Eluate concentration: up to 0.3–1 mg/ml
• Work time: 5 min at room temperature

Capturem Maxiprep Kits

• Isolate ~ 2.5 mg of your his-tagged protein with a purification system 
• Eluate concentration: up to 4.5 mg/ml
• Work time: 15 min at room temperature

Capturem His-Tagged Purification Large Volume

• Work time: 10–30 min at room temperature
• Yield: up to 25 mg/unit,
• Eluate concentration: up to 2.0 mg/ml 

Capturem His-Tagged Purification 96

• Elute concentration: up to 0.3–1 mg/ml
• Work time: just minutes, using vacuum filtration or centrifugation

2) COBALT AND NICKEL RESINS

His60 Ni Resin provided high binding capacity, which makes it an excellent choice for purifying large amounts of high-quality protein, while the Co²⁺ core of TALON resin ensures obtaining protein of very high purity. Both resins are available in broad range of formats.

1. Cobalt resins

TALON Single Step

• His-Tagged Protein Purification from Bacterial Cultures
• Complete extraction and purification in under 1 hour
• Purify target protein directly from bacterial culture
• Ready-to-use, prepacked columns
• Two sizes of columns available, for large- and small-scale purification

 HisTALON Superflow Cartridges

• Cobalt Resin FPLC Cartridges
• Easy-to-use prepacked 1 ml and 5 ml cartridges
• Maximize yield of biologically active protein
• Superior purity & high yields

HisTALON Gravity Columns

• Fast, Easy His-Tagged Protein Purification
• Easy-to-use prepacked gravity columns
• Maximize yield of biologically active protein
• Superior purity & high yields with TALON Resin

TALON Spin Columns

• Fast Small-Scale His-Tagged Protein Purification
• Exhibits high affinity for his-tagged proteins
• No copurification of proteins
• Resists metal leakage
• Performs well under a wide range of purification conditions
• Ready-to-use spin columns
• Each column contains 0.5 ml of resin

TALON Metal Affinity Resin

• Batch or Gravity Flow Purification of His-Tagged Proteins
• Exhibits high affinity for his-tagged proteins
• No copurification of proteins
• Resists metal leakage
• Performs well under a wide range of purification conditions

TALON CellThru

• His-Tagged Protein Purification from Crude Cell Lysates
• Purification from crude cell lysate
• Ideal for purification of membrane-bound protein or multiprotein complexes
• No copurification of proteins
• Low metal ion leakage
• Fast & easy protocol

TALON Superflow Metal Affinity Resin

• FPLC Purification of His-Tagged Proteins
• Exhibits high affinity for his-tagged proteins
• Ideal for FPLC applications
• No copurification of proteins
• Resists metal leakage
• Performs well under a wide range of purification condition

TALON Magnetic Beads

• His-Tagged High-Throughput Micro-Scale Purification
• Quick and easy separation of his-tagged proteins
• Highly selective TALON chemistry for increased purity
• Eluted samples ideal for small volume applications

B. NICKEL RESINS

His60 Ni Resin

• His-Tagged Recombinant Protein Purification
• Up to 60 mg/ml of recombinant his-tagged protein
• Low metal ion leakage in eluted his-tagged protein
• For purification under native or denaturing conditions
• Use for batch/gravity flow and automated FPLC purification

His60 Ni Magnetic Beads

• His-Tagged High-Throughput Microscale Purification
• Quick and easy separation of his-tagged proteins
• Selective His60 Ni chemistry for increased purity
• Eluted samples ideal for small volume applications

His60 Ni Superflow Cartridges

• His-tagged protein purification on any LC system
• Up to 60 mg/ml of recombinant his-tagged protein (1 ml cartridge)
• Up to 300 mg/ml of recombinant his-tagged protein (5 ml cartridge)
• Low metal ion leakage in eluted his-tagged protein
• For purification under native or denaturing conditions
• Fast and reproducible processing on any LC system, including ÄKTA

His60 Ni Gravity Flow Columns

• His-Tagged Recombinant Protein Purification
• Obtain up to 60 mg/ml of recombinant his-tagged protein
• Low metal ion leakage in eluted his-tagged protein
• Purify under native or denaturing conditions
• Use for batch/gravity flow and automated FPLC purification