Mammalian Expression Systems

pHEK293 Ultra Expression Vectors

Expression in mammalian cells allows for proper protein folding and post-translational modification, features that are key for proper protein function in vitro. The pHEK293 Ultra Expression Vectors enable ultra-high, transient overexpression of a protein of interest in human HEK 293 cells. 

Main features:

• Exploits the HIV-1 TAR-Tat system to express a protein of interest at ultra-high levels
• Obtain up to 10 times higher protein production than with conventional CMV promoter driven expression systems

AVAILABLE VERSIONS:

pHEK293 Ultra Expression Vector I

• single plasmid for overexpression a protein of interest in HEK293 cells

pHEK293 Ultra Expression Vector II 

• includes the pHEK293 Enhancer Vector
• expression level of the target protein can be optimized by varying ratio of the two plasmids that are used for transfection