Lentiviral Transduction

Lentiviral systems provide stable and long expression. Both non-dividing and dividing cells can be infected as well as neurons without cytotoxic effects. Takara Bio provide wide selection of products from packaging systems, transduction enhancers, including novel Lenti-X Transduction Sponge, to titration kits and concentrators.

1) LENTIVIRAL PACKAGING

Lenti-X Packaging Single Shots

• Extremely simple and consistent one step method for producing high titer generating VSVg pseudotyped lentivirus
• The single tube format consists of individual vials containing lyophilized Xfect Transfection Reagent with Lenti-X lentiviral packaging plasmids

Lenti-X 293T Cell Line

• Special 293T clone selected for high titer lentivirus production.

2) PREMADE LENTIVIRAL PARTICLES

Viral particles for Tet-Inducible Gene Expression

• Ready made lentiviral particles to introduce constitutive expression of the Tet-On 3G transactivator in the cells
•  In the presence of doxycycline the Tet-On 3G transactivator binds and activates transcription of the P_TRE3G promoter

Viral particles for subcellular localization studies

• Prepackaged lentiviral particles
• Fluorescent proteins targeted to specific organelles or subcellular structures
• Suitable for creation of reporter cell lines for the study of cytoskeletal and organelle structure and function in living cells or protein localization studies

Viral particles for fluorescent whole-cell labeling

• Particles express a bright fluorescent protein: green (ZsGreen1), red (mCherry or tdTomato), yellow (ZsYellow1) or blue (AmCyan1)
• >90% transduction efficiency even at very low MOI
• Functional titer provided

3) TRANSDUCTION TOOLS

Lenti-X Transduction Sponge

• Microfluidic transduction without need of instrumentation
• Suitable even for cells difficult to transduce
• Minimized cell handeling and required volumes

Lenti-X Accelerator

• Magnetic bead based technology designed to accelerate lentiviral transduction experiments
• The workflow is complete in only 30 minutes and avoid using Polybrene
• Ideal for transducing polybrene-sensitive cells

Ecotropic Receptor Booster

• Used to temporarily elevate the density of the ecotropic receptor protein mCAT-1 on the surface of any target cell
• It allows to efficiently transduce human cells with ecotropic retrovirus or lentivirus that otherwise could only infect rodent cells
• It also increase the infection efficiency of any type of cells that are resistant to viral infection.

4) LENTIVIRAL CONCENTRATION AND TITRATION

Identification of integration sites

• Enables simple determination of lentivirus integration sites using GenomeWalker technology
• Compatible with most commercialy available lentivirus vectors

Lenti-X Maxi Purification Kit

• Produces high yields of purified virus from crude supernatants and concentrates the virus up to 10 fold
• It uses simple and gentle gravity flow protocol for more efficient and consistent lentiviral transduction and healthier target cells
• It also efficiently removes transduction inhibitors and cytotoxic contaminants

Lentivirus Titration

• Lenti-X GoStix Instant Titration test that get a yes or no in only ten minutesC
• Can determine when to harvest lentiviral supernatants only from 20 µl of the lentiviral supernatant
• The other titration kits offered by TaKaRa Bio are used based on methods like ELISA, qRT-PCR and qPCR

Lenti-X Concentrator

• Simple mix and spin protocol without need of ultracentrifugation
• It provides a fast, simple and efficient method for concentrating any lentiviral stock

5) VECTOR SYSTEMS

TaKaRa Bio offers a variety of highly optimized lentiviral vectors for many applications.

Lentiviral Vector Systems for Constitutive cDNA Expression

• Express the gene of interest from a strong CMV promoter
• Select for lentiviral integration using antibiotic selection

Bicistronic Lentiviral Vector Systems

• Coexpress the gene of interest and an antibiotic or fluorescent selectable marker from the same transcript
• Using IRES-containing lentiviral vector systems

Lentiviral Vector Systems with an EF-1 Alpha Promoter

• Express the gene of interest from an EF-1 alpha promoter in cell types
• CMV promoter can be silenced over time such as hematopoietic or stem cells

Lentiviral All-In-One Vector Systems for Inducible Expression of cDNA

• Tightly controlled, inducible expression clones of your gene of interest with only a single vector

Lentiviral Vector Systems to Control Protein Stability

• Clone the gene downstream of a ProteoTuner destabilization domain (DD)
• Control the stability of your protein with Shield1 a small molecule ligand which protects the DD fusion protein from being degraded

Lentiviral Vectors for Fluorescent Protein Expression

• Clone the gene in frame with one of many fluorescent proteins (FP)
• Monitor the expression and subcellular localization of the protein of interest