Adenoviral Transduction

Gene delivery process with adenoviral particles does not depend on cell-cycle and can be used to target primary as well as transformed cell lines. The adenovirus genome does not integrate into the genome of the target cells, so it works with rapidly dividing cells and slowly dividing and non-dividing cells.

1) ADENOVIRAL PACKAGING

Adenoviral Packaging Cells

• Low-passage transformed human embryonic kidney cell line for adenoviral production
• The highly transfectable
• It presents slower growth rate
• The cells are more strongly adherent
• Resistant to overconfluence compared.

2) VECTOR SYSTEMS

Adenoviral Expression System 3 

• The most advanced and the simplest adenoviral gene delivery system
• Available in multiple formats

Adeno-X Expression System 3 Tet-On 3G Inducible

• Combines the control of gene expression with the most advanced adenoviral vector system
• Very tight control of gene expression
• It is all-in one vector very easy to clone

3) LENTIVIRAL CONCENTRATION AND TITRATION 

Adeno-X Rapid Titer Kit

• Allows functional hexon-based adenovirus titration
• Assay takes 1–2 hours

Adeno-X qPCR Titration Kit

• Fast, simple and accurate method
• Functional titer in 4 hours
• For all Ad5-based adenoviral vectors using quantitative PCR

Adeno-X GoStix

• Instant test that strongly resembles the pregnancy test
• Qualitative measurement of adenovirus in 10 minutes
• Quick determination of optimal harvest time

Adenovirus Purification Kits

• Chromatography-based systems
• Enable to purify amplified adenovirus directly from the cell pellet
• Whole process done in less than 90 minutes
• Suitable for purification of adenovirus for both in vivo and in vitro studies

Adenovirus CAR Receptor Booster

• Renders cells that cannot be transduced with adenovirus
• Can be used to increase efficiency of adenoviral transduction in all cell types
• It consists of a vial of concentrated exosome-like vesicles
• Vesicles are coated with the coxsackie virus and adenovirus receptor protein
• Vesicles fuse with the plasma membrane and increase the level of the receptors on the cell surface