Isolation Kits for KingFisher Systems

IST Innuscreen kits are compatible with KingFisher ml and KingFisher Flex platforms. Kits were optimized for a variety of different starting materials. Automated tools for isolating high-quality nucleic acids are based on magnetic beads or unique Smart Modified Surfaces.

1) MAGNETIC BEAD BASED EXTRACTION

deltaPREP RNA Virus PLUS - KFFLX (MDX)

• Viral RNA extraction from various sample types
• CE-IVD certification
• Starting material: Cell-free body fluids & cell culture (incl. supernatant or medium), tissues, swabs, stool samples
• Extraction time: Approx. 37 minutes (without manual or internal lysis)

innuPREP AniPath DNA/RNA Kit 2.0 - KFFLX

• Extraction of bacterial DNA and viral DNA/RNA
• Ideally suited for veterinary diagnostics
• Starting material: Cell-free body fluids & cell culture (incl. supernatant or medium), blood (fresh or frozen), faecal samples, swabs, organ tissue
• Average yield: Depending on sample quality/quantity
• Extraction time: Approx. 60 min

innuPREP Blood DNA Kit - KFFLX

• Extraction of DNA from whole blood samples
• Starting material: Whole blood samples, fresh or frozen blood (stabilizers: EDTA or citrate)
• Average yield: Up to 10 µg DNA
• Extraction time: Approx. 37 minutes and lysis approx. 25 minutes

innuPREP RNA Virus PLUS Kit - KFFLX

• Viral RNA extraction from various sample types
• Starting material: Cell-free body fluids & cell culture (incl. supernatant or medium), tissues, swabs, stool samples
• Extraction time: Approx. 37 minutes (without manual or internal lysis)

innuPREP Virus DNA/RNA Kit – KFml

• For simultaneous extraction of viral DNA and RNA
• Starting material: Cell-free body fluids & cell culture (incl. supernatant or medium), tissue samples, swabs
• Extraction time: Approx. 30 minutes and lysis up to 60 minutes

innuPREP Virus RNA Kit - KFml

• Viral RNA extraction from various sample types
• Starting material: Cell-free body fluids & cell culture (incl. supernatant or medium), tissues, swabs, stool samples
• Extraction time: Approx. 30 minutes and lysis up to 60 minutes

2) SMART EXTRACTION

The method does not need phenol/chloroform, ion exchanger, silica materials, spin filter columns or magnetic particles.

Main features:

• Eliminates the need for centrifugation or magnetic instruments
• The whole process is performed in one SmartExtraction Tip (SE Tip)
• SE Tip contains a material with unique Smart Modified Surfaces which adsorbs genomic DNA
• Entire workflow is much faster and easier than using the conventional methods
• The unique extraction chemistry in combination with Smart Modified Surfaces is optimized to get maximum of yield and quality

innuPREP SE Blood direct HMW DNA Kit – KFFLX

• DNA extraction from whole blood samples

• Specifically designed for isolation of high molecular weight DNA
• Starting material: Whole blood samples, fresh or frozen blood (stabilizers: EDTA, heparin or citrate)
• Average yield: Depends on sample and used volume
• Extraction time: Approx. 56 minutes

innuPREP SE Blood&Eukaryotic Cells UHMW DNA Kit – KFFLX

• Extraction of DNA from cultivated eukaryotic cells or peripheral blood mononuclear cells
• Specifically designed for isolation of ultra-high molecular weight

• Starting material: Whole blood samples, fresh or frozen blood (stabilizers: EDTA or citrate), Eukaryotic cells
• Average yield: Depends on sample and used volume
• Extraction time: Approx. 60 min and lysis (depends on starting material)

innuPREP SE HMW DNA Kit - KFFLX

• Extraction of high molecular weight DNA from wide variety of samples
• Starting material: Tissue samples, rodent tails, yeast & bacterial cell culture or pellets (gram+ & gram-)
• Average yield: Depends on sample and used volume
• Extraction time: Approx. 60 minutes and lysis (depends on starting material)