naica® System for Crystal Digital PCR™

Main features:

• Fast time-to-result (under 4 hours) with less than 20 mins of hands-on time
• Possibility to run 1, 2 or 3 chips in one run to perfectly fit for the lab needs
• Droplet crystals created in chip chambers using patented microfluidic technology
• Images of crystals reveal the droplets that contain amplified targets in multiple channels
• 3-color or 6-color detection capability and multiplexing
  • Prism3: Blue, Green and Red channel (for e.g. FAM, VIC/HEX, Cy5 fluorophores)
  • Prism6: Blue, Teal, Green, Yellow, Red and Infrared channel (for e.g. FAM, Yakima Yellow, Atto550, ROX, Cy5, Cy5.5 fluorophores)
• Software enables automatic counts of droplet populations for conversion into absolute concentration of targeted DNA sequences in copies/µl.

Components of naica® system:

• Geode (sample partitioning and PCR)
• Prism (fluorescence reader)
• Crystal Miner software
• Single consumable chips: Sapphire and Ruby
• Reagents and kits, including oncology kits

Applications:

• Absolute quantification of DNA / RNA
• Multiplex analyses
• Copy number variation
• Rare event detection
• Liquid biopsy
• Gene expression
• NGS library calibration/ NGS results validation

 The naica® system workflow