Adenoviral Transduction

TaKaRa Bio

Adenoviral Packaging Cells

• The highly transfectable Adeno-X 293 Cell line is low-passage transformed human embryonic kidney cell line for adenoviral production
• It presents slower growth rate
• The cells are more strongly adherent and don´t die due to overconfluence compared with HEK 293.

 

Adenovirus Purification

• Adenovirus Purification Kits
• Chromatography-based sytems
• Systems enable to purify amplified andenovirus directly from the cell pellet in less than 90 minutes
• It is possible to use it for purification of adenovirus for both in vivo and in vitro studies

 

Adenovirus Titration

• Adeno-X Rapid Titer Kit
• Designed around a hexon-specific antibody and allows functional hexon-based adenovirus titration
• Adeno-X qPCR Titration Kit
• Provides a fast, simple and accurate method for titrating from all Ad5-based adenoviral vectors using quantitative PCR

 

Detection of Adenovirus

• Adeno-X GoStix
• Instant test that strongly resembles the pregnancy test
• From 20 µl of the adenoviral supernatant to determine if infectious virus was produced posttransfection and determine the optimum harvest time

 

Adenovirus CAR Receptor Booster

• The Booster renders cells that cannot be transduced with adenovirus
• Can be used to increase efficiency of adenoviral transduction in all cell types
• It consists of a vial of concentrated exosome-like vesicles
• Vesicles are coated with the coxsackie virus and adenovirus receptor protein
• Vesicles fuse with the plasma membrane and increase the level of the receptors on the cell surface

 

Adenoviral Vector Systems

• Adenovirus Expression Kit
• Viral expression system for mammalian cells
• Utilizes two different methods for recombinant adenovirus production
• Adenoviral Expression System 3 
• The most advanced and the simplest adenoviral gene delivery system
• Available in multiple formats
• Adeno-X Expression System 3 Tet-On 3G Inducible
• Combines the control of gene expression with the most advanced adenoviral vector system
• It is all-in one vector very easy to clone

 

Documents and resources

For more information visit producer's website.