Bacterial Expression Systems

TaKaRa Bio

The pET Expression System

• Higher inducible protein expression levels and tighter control of your target gene
• E. coli BL21(DE3)-based system
• Choose between N- & C-terminal 6xHN-tagged vectors
• Choice of fast, simple In-Fusion PCR cloning or traditional T4 DNA ligase cloning
• Purify your expressed protein with His60 Nickel resin or TALON Cobalt resin

 

pCold Cold Shock Expression System

• Expression of difficult proteins that can not be expressed with the T7 system
• Increased solubility due to expression at reduced temperature
• Increased purity due to repressed expression of host proteins
• When used in conjunction with one of Takara's Chaperone Plasmids, the amount of recoverable soluble protein can be further increased
• Up to 90% of newly expressed cellular protein is labeled target protein

 

Single Protein Production (SPP) System

• Convenient for radioisotope labeling
• Up to 90% of newly expressed cellular protein is labeled target protein
• NMR analysis
• Purified protein expression

 

Brevibacillus Expression System

• Efficient production of secreted or intracellular recombinant target proteins
• Negligible amounts of extracellular protease - products remain intact in culture medium
• Unlike E. coli, no endotoxins
• Proteins are produced in active form
• Easy to culture, handle and sterilize

 

B. subtilis Secretory Protein Expression System

• Includes pBE-S DNA, an E. coli/B. subtilis shuttle vector containing
  • B. subtilis-derived subtilisin (aprE) promoter
  • Secretory signal peptide (aprE SP)
  • Multiple cloning site (MCS)
  • 3' (C-terminal) His-tag sequence
• Supplied with SP DNA Mixture, a library of DNA sequences encoding 173 unique secretory signal peptides 
• Fully compatible with In-Fusion cloning kits and systems, allowing rapid and easy construct generation
• Includes B. subtilis strain RIK1285

 

​Documents and resources

For more information visit producer's website.