Baculovirus Expression Sysrems

TaKaRa Bio

• Uses the baculovirus Autographa californica nuclear polyhedrosis virus (AcMNPV) to produce target proteins in insect cells.
• The target gene is inserted into a shuttle vector, which is cotransfected into insect host cells with the linearized BacPAK6 Viral DNA.
• The specially designed BacPAK6 Viral DNA forces recombination between the virus and transfer vector, resulting in high recombination efficiency.
• Following recombination, a few viral plaques are picked and purified, and the recombinant phenotype is verified.
• The newly isolated recombinant virus can then be amplified and used to infect insect cell cultures to produce large amounts of the desired protein.

 

 

Documents and resources

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