Chemiluminescent Reporters

TaKaRa Bio

Beta-Galactosidase Assay and LacZ Vectors

The beta-galactosidase assay is commonly used as a reporter, to monitor transfection efficiency, and for promoter/enhancer studies.

Colorimetric assay

• The Beta-Galactosidase Staining Kit
• Complete system for colorimetric detection of beta-galactosidase expression in mammalian cells

Chemiluminescent assay

•  The Luminescent Beta-Galactosidase Reporter System 3
• Provide quantitative measurement of beta-gal activity using a chemiluminescent substrate

 

Flow chart of chemiluminescent beta-galactosidase assay

 

pBeta-Gal-Basic

• Eukaryotic promoter and enhancer sequences
• Serve as a negative control or to clone promoters for characterization

 

pBeta-Gal-Control

• Contains the SV40 promoter and enhancer sequences
• Can be used as a positive control or as a reference for comparing the activities of promoters and enhancers

 

pCMV-LacZ

• Constitutive mammalian reporter vector
• Can be used as a reference or control plasmid
• Expresses extremely high levels of beta-galactosidase from the human cytomegalovirus immediate early promoter (PCMV IE) 

 

Luciferase assay

In Vivo Luciferase Imaging Kit

• Provides a bioluminescent assay
• Simplifies the analysis of promoter function in live cells or live animals
• Fast and easy—detect promoter activity in live cells and live animals
• For live-cell and live-animal imaging
• Allows repeated assaying over time

 

Ready-To-Glow Secreted Luciferase Assay

• Based on the secreted Metridia luciferase reporter.
• Combines the advantages of a live-cell assay with the sensitivity of an enzyme-based system
• Secreted luciferase assay protocol eliminates cell lysis
• 2–4 fold higher signal than firefly or Renilla luciferase
• Single transfection allows multiple time points from same cell

 

ProLabel

ProLabel Chemiluminescent Assay

• Provide sensitive, chemiluminescence-based assays
• For detecting and measuring the expression of any recombinant protein of interest
• Western blot kit alternative: highly sensitive, easy to use
• A quantitative enzymatic assay that’s simple and fast
• High-throughput compatible

 

Secreted Alkaline Phosphatase

Secreted Alkaline Phosphatase Assay

• The Great EscAPe SEAP 2.0 System uses a secreted alkaline phosphatase assay
• For analysis cis-acting DNA sequences and trans-acting factors
• One step assay, for monitoring promoter activity
• 10-fold higher sensitivity then firefly luciferase
• No-cell-lysis protocol

 

Documents and resources

For more information visit producer's website.