DNA Manual Extraction

IST Innuscreen GmbH

IST Innuscreen offers an extraordinary wide range of purification and isolation kits for nucleic acids from different starting materials. There are two main types of spin filter kits with Dual Chemistry Technology:

• innuPREP Kits - standard products for the isolation and purification of nucleic acids
• blackPREP Kits - optimized for complex starting material

 

innuPREP DNA Kit 

• The innuPREP DNA Kit represents a fast and effective means of purifying genomic DNA from wide variety of starting materials.
• Spin Filter columns used for isolation
• Patented DC-Technology for faster lysis and highly efficient gDNA binding
• InnuPREP DNA Mini Kit 2.0
• InnuPREP DNA Micro Kit
  • Specially optimized to accommodate small starting quantities

 

innuPREP DNA/RNA Mini Kit

• For rapid, parallel extraction of genomic DNA and total cellular RNA from a single starting sample
• Flexible for use with different starting materials
• Also for eucaryotic cells, gram+ and gram– bacteria and tissue samples
• Ready-to-use DNA and RNA in just 15 – 40 minutes
• Easy to use, no toxic β-mercaptoethanol

 

 innuPREP Blood DNA Mini  Kit

• Highly efficient tool for directly isolating DNA from whole blood samples
• High-quality isolated DNA that can be used immediately for photometric determinations, PCR or other downstream applications
• 2 protocols: < 200 μl and up to 400 μl of blood samples
• Excellent purity and yields of up to 30 µg
• deltaPREP Blood DNA Kit (MDX)
  • CE-IVD certified

 

innuPREP Plant DNA Kit

• For quickly and easily isolating DNA from an extremely wide variety of plant starting materials
• Isolation of highly pure DNA free of plant inhibitors and secondary metabolic products
• High yields from up to 100 mg starting material
• 3-lysis buffer system for optimized and specialised lysis of plant material

 

innuPREP Bacteria DNA Kit

• Bacterial DNA extraction from gram+ and gram–cell cultures
• Patented DC-Technology for short lysis times and efficient binding of bacterial DNA
• DNA isolation from up to 1 × 10⁹ bacterial cells

 

innuPREP Stool DNA Kit

• For isolating bacterial or genomic DNA from human or animal stool samples
• Includes prefiltration for removing undissolved sample components
• Ready-to-use DNA in just 30 – 45 minutes, free of inhibitors and other impurities

 

innuPREP Virus DNA/RNA Kit

• Simultaneous isolation of viral DNA and RNA from a variety of starting materials
• Extraction method based on the use of Spin Filters
• Optimum removal of inhibitors ensures trouble-free use of nucleic acids in subsequent applications
• Recommended for samples with unknown virus
• Includes Carrier Mix with internal DNA and RNA extraction control

 

innuPREP MP Basic Kit A

• Fast, efficient isolation of DNA and RNA of either viral or bacterial origin
• Based on manual magnetic particle separation with various magnetic racks
• Optimized for a variety of different starting materials and quantities
• Positive test results for an extremely wide range of viruses and bacteria

 

innuPREP Forensic Kit

• Optimal DNA extraction from very small forensic or highly contaminated samples
• Ready-to-use genomic DNA for immediate downstream application
• Large range of starting materials have been tested with positive results
• Certified for in-vitro diagnostic applications (CE-IVD)

 

innuPREP Plasmid Kit 

• Optimized for parallel extraction of plasmid DNA
• Application-specific Lysis Tubes with beads guarantee effective homogenization of the starting material
• Patented extraction chemistry with a stringent Lysis Buffer system and a novel Binding Buffer
• When combined with rapidSTRIPE assays, the kit serves as a highly sensitive tool for detecting tick-borne pathogens (including TBE and/or FSME)
• innuPREP Plasmid Mini Kit 2.0
  • Allows for processing of 0.5 ml to 15 ml of bacterial cultures
  • ​New filter membrane allows isolation of up to 80 µg pDNA from one sample
• innuPREP Plasmid Midi Kit
  • Designed for the extraction of plasmid DNA from up to 100 ml of cultured bacterial cells​​
  • Excellent quality plasmid DNA is suited for a lot of downstream applications like transfection, cloning, sequencing, PCR or in vitro transcription
  • Efficient endotoxin removal procedure with DNA precipitation

 

innuPREP Sewage Water DNA/RNA Kit

• Designed for the isolation of DNA and RNA from sewage water samples
• Nucleic acid extracted from viruses, bacteriophages and free-circulating DNA/RNA
• Extraction from sewage sediment, from the corresponding supernatant and simultaneously from the sewage sediment and from the corresponding supernatant
• Extraction process starts with 20 ml of sewage water sample

 

blackPREP Rodent Tail DNA Kit

• Optimized for extracting genomic DNA from pieces of rodent tails (complex starting materials)
• Extremely fast lysis and highly efficient DNA isolation in just 1 and no more than 3 hours
• Samples do not need to be incubated overnight
• Mouse and rat tail pieces can be up to 1.2 cm and 0.6 cm in length, respectively

 

blackPREP FFPE DNA Kit

• Isolation of highly pure genomic DNA from FFPE tissue samples
• Extra short lysis time by using special Lysis Buffer
• No deparaffinization is needed
• Without use of hazardous or toxic components like phenol, octane or xylene
• High yield and optimal quality in less than 2.5 h
• Suitable for many kinds of tissue samples
• High quality nucleic acids for direct use or storage
• Excellent DNA integrity
• For in-vitro diagnostic use (CE-IVD)

 

blackPREP Food DNA I Kit

• Extremely fast isolation of bacterial DNA, esp. from food cultured in a Stomacher apparatus
• Established Spin Filter column technology makes the system easy to use
• Only 1 h required for extraction (including lysis)
• Highly sensitive detection of food pathogens when used in combination with rapidSTRIPE assays

 

blackPREP Tick DNA Kit

• Optimized for extracting pathogen DNA from ticks
• Includes application-specific Lysis Tubes with homogenization beads
• When combined with rapidSTRIPE assays, the kit provides a highly sensitive means of detecting tick-borne pathogens
• Based on well-established Spin Filter technology

 

blackPREP Tick DNA/RNA Kit

• Flexible combination kit for isolating high copy and low copy plasmid DNA, P1 constructs etc.
• Allows preparation of a wide volume range between 0.5 ml to 15 ml bacterial culture
• Purification of up to 80 µg high qualitative plasmid DNA
• Optimized Spin Filter membranes with adapted binding capacity
• Accelerated routines thanks to optimized protocols
• Direct use of high qualitative plasmid DNA for sensitive applications, like transfection, cloning, PCR or sequencing

 

blackPREP Swab DNA Kit

• Optimized protocol; chemistry adapted for DNA isolation from buccal swabs
• Fast and easy to use: DNA in just 20 – 25 minutes
• Prefiltration step maximizes DNA yields
• Includes high-quality swabs for sampling

 

innuSPEED Soil DNA Kit

• A tool for extracting microbial DNA from soil samples
• Combination of rapid homogenization with efficient digestion of microbial cell walls using specially developed Lysis Tubes
• A novel wash buffer efficiently removes inhibitors such as humic acids

 

smart DNA prep (m) kit

• Extraction of genomic DNA from various tissue samples, rodent tails, eukaryotic cells, bacteria and yeasts
• Based on the novel SmartExtraction technology
• Lysis, binding, washing and elution are efficiently performed in a simple thermal shaker
• Extracted nucleic acids are of superior quality and very high molecular weight

 

smart Blood DNA Midi prep (m)

• Based on manual application of SmartExtraction technology
• Up to 10 ml whole can be prepared
• 3-step-lysis consisting of lysis of erythrocytes, collection and digest of DNA carrying cells, DNA is efficiently bound, washed and eluted
• Minimal shearing power for purification of high molecular weight DNA up to 500 kbp

 

innuPREP SE HMW DNA Kit (m)

• Designed for the manual isolation of high molecular weight (HMW) genomic DNA (200 kb – 500+ kb)
• Extraction from tissue samples, eukaryotic cells, rodent tails, bacteria and yeasts samples using unique SmartExtraction Tube (SE Tube)
• Simple to handle extraction proces

 

innuPREP SE Blood&Eukaryotic Cells UHMW DNA Kit (m)

• Designed for manual isolation of high molecular weight (HMW) and ultra-high molecular weight (UHMW) genomic DNA
• Suitable for extraction from eukaryotic cells or peripheral blood mononuclear cells (PBMC) derived from fresh or frozen blood stabilized with EDTA, citrate or heparin
• No centrifugation – easy extraction using thermal shaker and magnetic rack
• High yield of ultra high-molecular-weight DNA

 

Documents and resources

For more information visit the producer's website.

Brochure Product Guide Extraction