DYKDDDDK-Tagged Protein Purification

TaKaRa Bio

The DYKDDDDK or FLAG tag is one of the most commonly used hydrophilic octapeptide tags. This tag is ideal for identifying and monitoring expression of DYKDDDDK- or FLAG-tagged proteins. Anti-DYKDDDDK beads are used to purify or immunoprecipitate DYKDDDDK-tagged or FLAG-tagged fusion proteins from cell lysates.

 

Immunoprecipitation Buffer Set

• Contains a highly optimized mix of buffers provided extremely low background
• Recommended for use with anti-DYKDDDDK beads

 

Magnetic Beads Immunoprecipitation Buffer Set

• Contains lysis, wash, and elution buffers optimized for purifying proteins
• Immunoprecipitation with magnetic beads, such as our Anti-Myc Magnetic Beads

 

Anti-DYKDDDDK Antibody

This monoclonal antibody recognizes the well-known FLAG epitope tag and detects N- or C-terminally tagged FLAG fusion proteins on Western blots.

• Highly specific antibody does not cross-react with endogenous proteins
• Higher sensitivity than competitor’s anti-FLAG antibody conjugated beads
• Use beads to purify or immunoprecipitate FLAG-tagged fusion proteins from cell lysates
• Recommended for
  • Western blotting
  • Immunofluorescent staining
  • ELISA
  • Protein purification
  • Immunoprecipitation with beads

 

pCMV-DYKDDDDK Vector Set

Mammalian expression vectors allow to express protein of interest fused to DYKDDDDK tag (FLAG epitope tag). The DYKDDDDK or FLAG epitope is well characterized, highly immunoreactive and thus is ideal for co-immunoprecipitation studies.

• The DYKDDDDK or FLAG tag is one of the most commonly used hydrophilic octapeptide tags
• N- and C-terminal vectors are ideal for cloning and expression of DYKDDDDK- or FLAG-tagged proteins
• Highly specific antibody does not cross-react with endogenous proteins
• Use beads to purify or immunoprecipitate FLAG-tagged fusion proteins from cell lysates

 

Documents and resources

For more information visit producer's website.