Automated Isolation Kits for Cybio FeliX

Optimized pipetting steps for maximum purity of eluted nucleic acids. Samples can be used directly in subsequent applications, e.g. qPCR. Kits based on Magnetic Beads or Smart Extraction technology, available usually in 96 or 480 reactions format. 

1) MAGNETIC BEADS BASED EXTRACTION 

To reduce the manual preparation steps for these kits to a minimum, the innuPREP Prefilling Set is available for automated prefilling of deep well plates with the appropriate buffers and reagents by CyBio FeliX Extraction Set.

innuPREP Plant DNA Kit-FX

• Isolation of DNA from plant material
• Effective removal of inhibiting by-products (e.g. secondary metabolites)
• Starting material: Plants (fresh or frozen material)
• Extraction time: Lysis – 45 minutes; Extraction – 26 minutes

innuPREP Blood DNA Mini Kit-FX

• Isolation of genomic DNA from whole blood
• Protocols for 200 μl and 400 μl of sample
• Starting material: Blood (fresh, frozen)
• Extraction time: 70 min (200 μl) or 86 min (400 μl)

innuPREP AniPath DNA/RNA Kit-FX

• Extraction of bacterial DNA and viral DNA or RNA in parallel
• Starting material: Plasma, serum, whole blood, fecal samples or swabs, organ tissue (spleen, brain, lungs, etc), cell culture supernatant
• Extraction time: Lysis – depending on starting material, Extraction – 86 min

innuPREP FFPE DNA Kit-FX

• Extraction of genomic DNA from FFPE (tissue samples)
• Novel chemistry for complete elimination of paraffin removal (no toxic solvents)
• Starting material: FFPE (formalin fixed, paraffin embedded) tissue
• Extraction time: Lysis - approx. 120 min (external), Extraction - 54 min

innuPREP Food DNA I Kit-FX

• Automated isolation of DNA from a stomacher cultivation
• Extraction time: With pre-lysis - 108 min, without pre-lysis - 75 min

innuPREP Virus DNA/RNA Kit-FX

• Isolation of virus nucleic acids from cell-free serum or plasma
• Preparation from 200 μl and 400 μl of sample
• Starting material: Serum, plasma
• Extraction time: 62 min (200 μl) or 73 min (400 μl)

innuPREP Virus TS RNA Kit 2.0-FX

• Specially developed for SARS-CoV-2 RNA isolation from swabs
• Kit includes carrier RNA for maximum yields
• Starting material: Swab samples (nasopharyngeal and oropharyngeal)
• Extraction time: 62 min

2) SMART EXTRACTION

The method does not need phenol/chloroform, ion exchanger, silica materials, spin filter columns or magnetic particles.

Main features:

• Eliminates the need for centrifugation or magnetic instruments
• The whole process is performed in one SmartExtraction Tip (SE Tip)
• SE Tip contains a material with unique Smart Modified Surfaces which adsorbs genomic DNA
• Entire workflow is much faster and easier than using the conventional methods
• The unique extraction chemistry in combination with Smart Modified Surfaces is optimized to get maximum of yield and quality

smart DNA prep (a96)-FX

• Universal kit for tissue samples, eukaryotic cells, bacteria and yeast cells
• Up to 500 µl elution volume
• Starting material: Tissue, eukaryotic cells, yeast & bacterial cell culture or pellets (gram+ & gram-)
• Extraction time: External lysis – depends on material, Extraction – 40 min

smart Blood DNA Midi prep (a96)-FX

• DNA isolation from PBMC's from 0.5 to 3 ml of fresh or frozen whole blood
• Average yield: from 5 up to 90 μg
• Starting material: Blood (stabilizers: EDTA, citrate and heparin, PAXgene Blood DNA Tube)
• Extraction time: External lysis - 40 min, Extraction - 40 min

smart Blood DNA Midi Direct prep (a96)-FX

• DNA isolation from PBMC's from 0.2 to 1 ml of whole blood or 1 ml of buffy coat
• Average yield: from 5 up to 40 μg (blood) or 30 to 60 μg (buffy coat)
• Starting material: Fresh/Frozen blood (stabilizers: EDTA, citrate and heparin, PAXgene Blood DNA Tube)
• Extraction time: 90 min (500 μl) or 120 min (1000 μl)

smart Plant DNA prep (a96)-FX

• DNA isolation from plant material
• Elimination of interfering plant metabolites and possible PCR inhibitors
• Starting material: Plants (fresh or frozen material), including leaves, roots, seeds and more
• Extraction time: Lysis - depending on the material, Extraction - 40 min