cDNA Synthesis

1. PrimeScript RTase

This recombinant and modified MMLV reverse transcriptase is efficient for preparation of cDNA up to 12 kb in length. PrimeScript is suitable for all applications where full-length DNA is required including cDNA library preparation or RT-qPCT.

Main Features:

• Works on challenging templates (GC rich templates and templates with high levels of secondary structure)
• Capable of extending up to 12 kb templates with minimal background
• cDNA synthesis and extension through complex RNA structure at 42 °C
• High specificity: reduced mispriming increases reverse transcription efficiency
• High accuracy reverse transcription

STANDARD VERSIONS:

PrimeScript™ Reverse Transcriptase

• For full-length cDNA synthesis (using your own primers)
• PrimeSctript is supplied as enzyme and 5X PrimeScript Buffer in separate tubes

 PrimeScript First Strand cDNA Synthesis Kit

• Kit full-length cDNA synthesis primers included in the kit
• Full kit with all reagents needed including Oligo dT Primer and Random 6-mers

PrimeScript™ IV 1st strand cDNA Synthesis Mix

• Improved for cDNA synthesis from small total RNA amounts
• Supplied as PrimeScript IV enzyme mix and random 6-mers in separate tubes

PrimeScript reverse transcriptase is also available as RT-PCR kits. For more information see RT-PCR kits.

2. PrimeScript II

PrimeScript II reverse transcriptase is suitable for long cDNA synthesis or long reverse transcription of RNA with high levels of secondary structure. Also, non-specific extension is inhibited by accessory protein.

Main Features:

• MMLV RNase H minus RTase with strong extension activity
• Rapid cDNA synthesis and extension at 42 °C
• Includes accessory protein
• Suitable for high-throughput: robust yield even after samples are pre-incubated on ice
• Higher yield during cDNA synthesis of long products relative to original PrimeScript RT

STANDARD VERSIONS:

PrimeScript™ II Reverse Transcriptase

• Supplied as enzyme and 5X PrimeScript II Buffer in separate tubes, accessory protein included

PrimeScript II 1st Strand cDNA Synthesis Kit

• 1st strand cDNA synthesis from total or poly(A)+ RNA with minimal background
• Kit contains all the necessary reagents

3. SMARTScribe

This modified MMLV reverse transcriptase performs unbiased cDNA synthesis. SMARTScribe generates long, full-length cDNA (up to 14.7 kb), but relative transcript proportions of the original RNA sample remain preserved.

Main Features:

• Synthesize long, full-length cDNA up to 14.7 kb
• Amplify rare transcripts
• Maintain the complexity of the original RNA

STANDARD VERSIONS:

SMARTScribe™ Reverse Transcriptase

• Includes SMARTScribe RT, 5X First-Strand Buffer and 20 mM DTT

4. SMART MMLV RT

SMART MMLV Reverse Transcriptase is an ultra-pure, recombinant Moloney Murine Leukemia Virus reverse transcriptase (MMLV RT). It helps prevent degradation of template RNA and still allows the synthesis of high quality, full-length cDNA (up to 11.7 kb) from almost any transcript.

Main Features:

• Synthesize long, full-length cDNA up to 11.7 kb
• Amplify rare transcripts
• Maintain the complexity of the original RNA
• Robust ultrapure MMLV with consistent performance

STANDARD VERSIONS:

SMART® MMLV Reverse Transcriptase

• SMART® MMLV Reverse Transcriptase is supplied with5X First-Strand Buffer and 100 mM DTT in separate tubes

5. MMLV RT, GPR

This ultra-pure recombinant MMLV reverse transcriptase is a registered general purpose reagent (GPR). MMLV RT, GPR is fully active RT that is free of exogenous RNases and other nucleases, so it prevents template RNA degradation. It allows synthesis of long, full-length cDNA of up to 11.7 kb in length.

Main Features:

• Registered as general purpose reagents (GPR)
• High Lot-to-lot reproducibility, consistency, and a wide dynamic range
• Enzyme purity >90% as assayed by microfluidic chip
• Synthesize long, full-length cDNA up to 11.7 kb

• Robust, ultra-pure MMLV RT with consistent performance

STANDARD VERSIONS:

MMLV Reverse Transcriptase, GPR

• Supplied as enzyme, 5X Reverse transcription Buffer and DTTs in separate tubes

6. AMV Reverse Transcriptase XL

AMV Reverse Transcriptase XL enables synthesis of full-length cDNA from single-stranded RNA, DNA, or RNA-DNA hybrid templates. It can be used over a temperature range of 37–55°C, but with optimal temperature between 41–44°C. It can be used for full-length cDNA synthesis from templates of up to 10 kb in length.

Main Features:

• Synthesize long, full-length cDNA up to 11.7 kb
• Requires a primer to initiate synthesis and Mg²⁺ or Mn²⁺ for activity
• Get full-length cDNA from single-stranded RNA, DNA, or RNA-DNA hybrid templates
• It has 5→3' ribonuclease H activity

STANDARD VERSIONS:

AMV Reverse Transcriptase XL for RT-PCR

• Supplied as a full kit with all reagents included