Manual DNA Extraction

IST Innuscreen offers an extraordinary wide range of purification and isolation kits for nucleic acids from different starting materials. Spin filter-based, meal bead-based of smart extraction technology-based kit available.

1) SPIN FILTER-BASED KITS

innuPREP DNA Mini Kit 2.0

• Fast genomic DNA extraction
• Starting material: Blood, fresh or frozen (RNA only fresh), eukaryotic cells, tissue, rodent tails, yeast & bacterial cell culture or pellets (gram+ & gram-), swabs
• Average yield: Up to 60 µg
• Extraction time: Approx. 8 minutes after lysis

deltaPREP Blood DNA Mini Kit (MDX)

• Specifically designed for DNA from whole blood samples of up to 400 μl
• CE-IVD certification
• Starting material: Blood, fresh or frozen (RNA only fresh)
• Average yield: Up to 30 µg
• Extraction time: Approx. 24 minutes, including lysis

innuPREP Blood DNA Mini Kit

• Extraction of DNA from whole blood samples of up to 400 μl
• Starting material: Whole blood samples (up to 400 µl), fresh or frozen blood (stabilizers: EDTA, heparin or citrate)
• Average yield: Up to 30 µg
• Extraction time: Approx. 24 minutes, including lysis

blackPREP FFPE DNA Kit

• Optimised for fast extraction of genomic DNA from FFPE samples
• Without usage of toxic solvent
• No deparaffinisation step needed
• Starting material: FFPE (formalin-fixed, paraffin embedded) tissue
• Average yield: Depends on the type and starting quantity of sample
• Extraction time: Approx. 2.5 hours (incl. lysis)

blackPREP Rodent Tail DNA Kit

• For DNA extraction from pieces of mouse and rat tails
• Starting material: Rodent (mouse or rat) tails
• Average yield: Mouse tail pieces (1.2 cm): 30 – 40 µg, Rat tail pieces (0.6 cm): 35 – 45 µg
• Extraction time: Approx. 9 minutes
• Lysis time: Between 1 and 3 hours max.

innuPREP Bacteria DNA Kit

• DNA extraction from Gram-positive and Gram-negative bacteria cells
• Starting material: Up to 1 x 10⁹ cultured pellets of Gram-positive and Gram-negative bacteria
• Average yield: Depends on sample and used volume
• Extraction time: Approx. 15 minutes after lysis step

innuPREP Forensic Kit

• gDNA extraction from tiny samples and highly contaminated forensic specimens
• Starting material: Forensic material (bone meal, chewing gum, cigarette bulbs, nails, hairs etc.)
• Average yield: Depends on the type and quantity of the sample used
• Extraction time: Approx.15 minutes after lysis

blackPREP Tick DNA/RNA Kit

• Simultaneous isolation of DNA and RNA from ticks
• Starting material: Ticks
• Average yield: Depends on the type and quantity of the sample used
• Extraction time: Less than 1 hour (including lysis)

innuPREP DNA/RNA Mini Kit

• Simultaneous isolation of DNA and RNA from single sample
• Starting material: Tissue, eukaryotic cells, yeast & bacterial cell culture or pellets (gram+ & gram-)
• Average yield: Up to 60 µg RNA; up to 40 µg DNA
• Extraction time: 15 – 40 minutes

innuPREP Plant DNA Kit

• Extraction of DNA from an extremely wide variety of plant materials
• Starting material: Various plant materials (fresh, frozen or dried plant material)
• Average yield: approx. 3 – 25 µg
• Extraction time: approx. 30 – 40 minutes

innuPREP Plasmid Mini Kit 2.0

• Fast and easy isolation of plasmid DNA from bacterial cultures
• Starting material: Yeast & bacterial cell culture or pellets (gram+ & gram-)
• Average yield: Typical yield from 2 ml (high copy Plasmid): approx. 6 – 20 µg
• Extraction time: Approx. 15 min

innuPREP Stool DNA Kit

• Extraction of bacterial DNA from stool samples
• Isolation genomic DNA from shedded intestinal epithelial cells
• Starting material: Stool samples (human or animal origin)
• Average yield: Depends on the type and quantity of the starting material
• Extraction time: Approx. 30 – 45 minutes

innuSPEED Soil DNA Kit 2.0

• Microbial DNA extraction from soil samples
• Starting material: Soil
• Average yield: Up to 60 µg
• Extraction time: 30 minutes of homogenization and lysis and approx. 16 minutes purification

2) MAGNETIC BEAD BASED EXTRACTION

innuPREP MP Basic Kit A

• Bacterial/Viral DNA and RNA extraction from from various cell-free body fluid
• Starting material: Serum, plasma, saliva, synovial fluids, cell culture (incl. supernatant or medium), swabs, stool samples
• Extraction time: Approx. 20 min. after lysis

innuPREP Sewage Water DNA/RNA Kit

• Isolation of DNA and RNA from sewage water samples
• Extraction from: Viruses, bacteriophages and free-circulating DNA/RNA (e.g. plasmid-associated DNA coding for bacterial resistances) in water
• Starting material: Sewage water (supernatant and the sediment alone or both combined could be used for extraction)
• Extraction time: 60 min. including all steps

3) SMART EXTRACTION

The method does not need phenol/chloroform, ion exchanger, silica materials, spin filter columns or magnetic particles.

Main features:

• Eliminates the need for centrifugation or magnetic instruments
• The whole process is performed in one SmartExtraction Tube (SE Tube)
• SE Tube contains a material with unique Smart Modified Surfaces which adsorbs genomic DNA
• Entire workflow is much faster and easier than using the conventional methods
• The unique extraction chemistry in combination with Smart Modified Surfaces is optimized to get maximum of yield and quality

innuPREP SE Blood&Eukaryotic Cells UHMW DNA Kit (m)

• Isolation of high molecular weight (HMW) and ultra-high molecular weight (UHMW) genomic DNA
• High yield of ultra high-molecular-weight DNA
• Starting material: Blood, fresh or frozen (RNA only fresh), eukaryotic cells
• Average yield: Depends on sample and used volume
• Extraction time: Approx. 60 minutes after lysis

innuPREP SE HMW DNA Kit (m)

• Isolation of high molecular weight (HMW) genomic DNA (200 kb – 500+ kb)
• Works with various types of starting materials
• Starting material: Tissue, rodent tails, eukaryotic cells, yeast & bacterial cell culture or pellets (gram+ & gram-)
• Average yield: Depends on sample and used volume
• Extraction time: Approx. 60 minutes after lysis