Horizon Discovery CRISPR/Cas9 Systems
Customizable ready to use gene editing systems
Broad range of genome editing tools provided by Horizon Discovery (former Dharmacon) helps user to achieve any edit, in any cell type, at any scale. Choose form guide RNAs, Cas9 nuclease options or already engineered cell lines. With CRISPR Design Tool it is easy to generate custom site-specific guide RNAs.
1. Edit-R CRISPR guide RNA
Edit-R CRISPR guide RNAs can be purchased as pools or individual sgRNA in various formats. All predesigned Edit-R RNAs are guaranteed to edit targeted gene of interest. Positive, non-targeting and cutting controls are available to all RNA formats.
AVAILABLE FORMATS:
Synthetic single guide (sgRNA) or two part crRNA:tracrRNA
- Ready to be co-transfected with Cas9 mRNA or protein into the cells
- Compatible with electroporation
- crRNA needs to be used with synthetic tracrRNA
Lentiviral sgRNA
- Suitable for cells difficult-to-transfect
- Population enrichment with antibiotic resistance markers possible
Lentiviral All-in-one sgRNA + Cas9
- Complete solution, single reagent workflow
- Suitable even for difficult-to-transfect cells
- Population enrichment with antibiotic resistance markers possible
Custom design synthetic or lentiviral guide RNAs
- Tailored to needs of the experiment
- For additional species, alternative nucleases
2. Edit-R Cas9 nuclease
Horizon offers a variety of Cas9 nuclease solutions for CRISPR-based gene editing experiments.
AVAILABLE FORMATS:
Cas9 protein
- Suitable for electroporation or transfection together with sgRNA
- DNA-free, transient expression
Cas9 mRNA
- Suitable for electroporation or transfection together with sgRNA
- DNA-free, transient expression
- Enrich population with FACS
- Also available with fluorescent options
Lentiviral Cas9
- Available as particles or plasmid DNA
- Suitable even for difficult-to-transfect cells
- Gets stable cell lines
- Enrich population with resistance marker
"CRISPR-ready" premade Cas9-expressing cell lines
- Variety of popular cell backgrounds
- Ready for guide RNA delivery
