Illumina Library Construction

Takara Bio DNA-Seq kits works with complicated samples such as FFPE DNA or cfDNA and are designed for construction of libraries compatible with Illumina platforms. Kits are based on ThruPlex technology, which enables preparation of high quality library from as little as 50 pg of input material. In the portfolio kits for work with fragmented DNA (50 bp) and even for work with intact DNA or ssDNA are included.

Main features:

• Fast and simple workflow 3 steps in a single tube
• Library prepared in about 2 hours with 15 minutes hands on time
• Single tube protocol lowers the risk of contamination and sample loss
• Leftover adapters are degraded post-ligation – no intermediate cleanup needed
• Need to be paired with Unique dual index kit – multiplexing of up to 384 samples

 AVAILABLE KITS:

ThruPLEX DNA-Seq Kit

• High performance to generate DNA libraries from lowest input (50 pg to 50 ng)
• Can be used with fragmented DNA (such as gDNA, cfDNA, FFPE DNA)
• Can be used in any DNA-seq application, RNA-seq or ChIP-seq
• Validated with commonly used target enrichment platforms
  • Agilent SureSelect
  • Roche Nimblegen SeqCap EZ
  • IDT xGen Lockdown probes

ThruPLEX DNA-seq FLEX

• Inputs of 250 pg - 200 ng of fragmented dsDNA
• Suitable even for GC-rich regions
• Can be used with challenging samples (FFPE or cfDNA)

ThruPLEX Tag-seq FLEX

• Inputs of 250 pg - 200 ng of fragmented dsDNA
• Unique Molecula Identifiers (UMIs) are incorporated in the kit
• Improved analysis of low-frequency variants

ThruPLEX DNA-seq FLEX EF

• Inputs of 100 pg - 200 ng of intact dsDNA
• Size-tunable fragmentation module included
• Optimized even for GC-rich regions

ssDNA-Seq Kit

• Works with input amount of >10 pg dsDNA and ssDNA
• Optimized for fragments as short as 50 bp ssDNA
• Simple workflow finished in 2 hours
• Suitable for challenging samples like fragmented, damaged or degraded DNA