Immune Profiling

Highly sensitive approach to TCR and BCR sequencing

Takara Bio kits combine SMART (Switching Mechanism at 5' End of RNA Template) technology with 5' RACE to capture V(D)J sequences. With RNA as input material kits detect expressed receptor sequences of B-cell and T-cell receptors. Sequencing libraries can be multiplexed with up to 96 primer index options. Separate portfolio for human and mice is available.

Main features:

Designed for T-cell and B-cell receptor (TCR and BCR) profiling
Implementation of SMART and 5' RACE technologies
For detection of rare functional variants
Libraries are compatible with Illumina sequencing platforms
Work with wide range of input amounts
Multiplexing of libraries with up to 96 primer index options

AVAILABLE KITS:

1. HUMAN IMMUNE PROFILING KITS

SMART-Seq Human BCR (with UMIs)

Input: down to 10 ng of high-integrity RNA (may differ depending on starting material)
Sample material: PBMCs, B cells, spleen or bone marrow, and whole blood
Enables profiling of all heavy (IgG, IgM, IgA, IgD, IgE) and light (IgK, IgL) BCR chains
Can be miniaturized to reduce costs, or automated

SMARTer® Human BCR IgG IgM H/K/L Profiling Kit

Total RNA inputs: 10ng - 1 µg of RNA from blood or 1- 100 ng of RNA from B-cells
Accurate amplification of human IgG subclasses and identification
Enables flexible pooling strategies

SMART-Seq® Human TCR (with UMIs)

Input: 10 ng to 500 ng of total RNA or 1,000 to 10,000 purified T cells
Sample material: total RNA or 1000 -10,000 purified T-cells
Single primer pair for each TCR (alpha or beta) subunit per reaction
UMIs and UDIs implemented in the kit

SMARTer® Human scTCR a/b Profiling Kit

Starting material: FACS or manually sorted single cells
96 cells can be pooled into 12 libraries
Detection of low-abundance TCR variants

2. MOUSE IMMUNE PROFILING KITS

SMART-Seq® Mouse BCR (with UMIs)

Input: 10 ng–1 μg of total RNA (RIN>7)
Sample material: mouse spleen, bone marrow, or peripheral blood mononuclear cells (PBMCs)
For includes all heavy (IgG, IgM, IgA, IgD, IgE) and light (IgK, IgL) BCR chains
UMIs and UDIs re incorporated in the kit

SMARTer® Mouse BCR IgG H/K/L Profiling Kit

Input: 10 ng–3 µg of total RNA
Sample material: spleen, lymph node, PBMCs, and hybridomas
Generates data for both heavy (IgG only) and light chain diversity
Flexible pooling possible

SMART-Seq® Mouse TCR (with UMIs)

1 ng–1 µg of total RNA or 100–10,000 purified mouse T cells
Sample material: mouse spleen or T cells, mouse whole blood, bone marrow, or thymus
Either full-length V(D)J or CDR3 only can be sequenced
UMIs and UDIs re incorporated in the kit

SMARTer® Mouse TCR a/b Profiling Kit