naica® Consumables, PCR Mixes, and Kits

Specific consumables and PCR reagents for optimal performance of Crystal Digital PCR™ on the naica® and Nio™ systems

Chips:

Only a single pipetting step is required before processing in the instruments.

1) Sapphire

• 4 samples / chip
• Up to 30,000 droplets per sample
• Reaction volume 25 µl

2) Ruby

• 16 samples / chip
• Up to 17,000 droplets per sample
• Reaction volume 5 µl

PCR mixes:

Ready-to-use two-component mixes available in 5x and 10x concentration. PCR mixes do not include DNA primers, probes/intercalating dye and DNA template.

1) naica® multiplex PCR MIX 

• For optimal TaqMan fluorescent probe-based Crystal Digital PCR™
• Internal BLUE channel reference dye for partition detection and identification in the
• High multiplexing capacity
• Available for 150/750 (small) and 300/1500 (medium) reactions on Sapphire/Ruby chips

2) naica® PCR MIX 

• Optimised for EvaGreen fluorescent intercalating dye-based Crystal Digital PCR™
• Available for 300/1500 (medium) reactions on Sapphire/Ruby chips

Kits by Stilla Technologies:

1) naica® IQ/OQ Kit

• Ready-to-use kit for routine OQ
• Validated for use on both the naica® systems and Nio™ system
  • 24 reactions for two full OQ (using Sapphire Chips)
  • 96 reactions for one full OQ (using Ruby Chips)

2) EGFR 6-color Crystal Digital PCR™ Kit

• Analysis of up to 32 somatic EGFR mutations in NSCLC (exons 18, 19, 20, and 21)
• Ready-to-use kit, validated for 6-color naica® system
• 48 reactions on the Sapphire Chip
• For use with cell-free DNA (cfDNA) extracted from plasma samples