One-Step RT-qPCR

1. DYE-BASED DETECTION

One-Step TB Green PrimeScript RT-PCR Kit (Perfect Real Time)

This kit utalizes reverse transkriptase PrimeScript RTase and Takara Ex Taq HS DNA polymerase for RT-qPCR reaction in single tube. Premix contains TB Green intercalation dye for easy detection in real time.

Main features:

• One-Step RT-PCR lowers pipeting and contamination risks
• Uses PrimeScript RTase, a robust reverse transcriptase
• Includes Ex Taq HS, a high efficiency hot start PCR enzyme to detect small amount of RNA
• Optimized for One-Step RT-qPCR with TB Green detection

STANDARD VERSIONS:

One-Step TB Green® PrimeScript™ RT-PCR Kit (Perfect Real Time)

• Enzymes, buffer and ROX reference dye all supplied in separate tubes

One-Step TB Green® PrimeScript™ RT-PCR Kit II (Perfect Real Time)

• Higher specificity than previous version
• Enzymes premix, buffer and ROX reference dye all supplied in separate tubes

2. PROBE BASED DETECTION

One-Step PrimeScript III RT-qPCR Kit

2X concentrated mastermix combines heat resistant reverse transcription polymerase PrimeScript III RTase and Takara Taq HS DNA polymerase. Both, reverse transcription and qPCR can be run in single tube. Available also in 5x concentrated version with improved limit of detection.

Main features:

• Fast, easy one-step RT-qPCR in a single tube
• Probe-based detection
• No need for any preamplification
• Efficient, specific cDNA synthesis and amplification, even from complex RNA templates
• Suitable for a wide variety of samples (including crude RNA extracts)
• Compatible with automation systems
• Does not freeze

STANDARD VERSIONS:

One-Step PrimeScript™ III RT-PCR Kit

• Supplied as enzyme premix and ROX dye in separate tubes

One Step PrimeScript™ III RT-qPCR Mix, with UNG

• Supplied as enzyme premix and ROX dye in separate tubes
• Contains Uracil N-Glycosylase (UNG) to prevent carry over contamination

5X CONCENTRATED VERSIONS:

5X One Step PrimeScript™ III RT-qPCR Kit, no ROX, GPR

• 5X concentrated premix format, no ROX included in the mix

5X One Step PrimeScript™ III RT-qPCR Kit, ROX plus, GPR

• 5X concentrated premix format, ROX included in the mix

Lyo-Ready One Step PrimeScript™ III RT-qPCR Mix

• Designed to be ready for lyophilization process
• Supplied as 2X concentrated premix format

One-Step PrimeScript RT-PCR Kit

This complete kit utilizes PrimeScript reverse transcriptase and Takara Ex Taq PCR polymerase for RT-PCR reactions in sigle tube and single step. PrimeScript RTase is vieified to have low levels of RNase H and which exhibits an excellent extension rate and quick, full-length cDNA synthesis.

Main features:

• Easy: One-Step RT-PCR lowers pipeting and contamination risks
• Includes Ex Taq HS, a high efficiency hot start PCR enzyme
• Sensitive: accurate quantification of any RNA with TaqMan probe detection
• Robust and efficient
• PrimeScript (Rnase H Minus MMLV-based) RTase ensures efficient transcription from any RNA tempalte even with high secondary structure

OTHER AVAILABLE VERSIONS:

One Step PrimeScript™ RT-PCR Kit (Perfect Real Time)

• Enzymes premix, buffer and ROX reference dye all supplied in separate tubes

PrimeDirect Probe RT-qPCR Mix

Kit is designed for direct RT-qPCR reaction with probe detection. There is no need for nucleic acid extraction or purification prior the reaction. Kit is suitable for detection of pathogens, including viruses and bacteria, in various biological specimens and to directly analyze gene expression in cells.

Main features:

• One-step RT-qPCR premix for ease of use
• Strong thermostability
• High tolerance for PCR inhibitors (e.g., heparin from blood and humic acid from soil)
• Compatible with direct addition of crude extracted RNA or samples to reaction mix
• Suitable for all probe-based assays

STANDARD VERSION:

PrimeDirect™ Probe RT-qPCR Mix

• Supplied as a 2X premix ROX reference dye all supplied in separate tubes

PrimeDirect™ Probe RT-qPCR Mix, with UNG

• Supplied as a 2X premix ROX reference dye all supplied in separate tubes
• Contains Uracil N-Glycosylase (UNG) to prevent carry over contamination