Preamplification reagents

Preamplification can help overcame the challenges, which enables enables unbiased, target-specific amplification of cDNA and gDNA. When working with limited or precious starting materials, such as nucleic acids extracted from FFPE tissue, samples obtained in extremely small quantities, or material that occurs at dilute concentrations in biofluids, preamplification can provide good results.

Prelude One-Step PreAmp Master Mix

Optimized reverse transcriptase, polymerase, and buffer in Prelude One-Step PreAmp MasterMix maximize RT-PCR efficiency for all targets. It ensures preamplification of >100 targets, from as little as 100 pg mRNA or 10 ng total RNA. Amplification products are suitable for other applications such as real-time PCR (qPCR), genotyping, or target-enrichment analysis.

Main features:

• Ideal for a limited (10 pg–100 ng) input of cDNA or gDNA
• 2X PCR mix optimized for preamplification of multiple (>100) targets
• Ready-to-use master mix
• Suitable for work with FFPE tissue, biofluids, laser capture microdissection or exosomes
• One-Step preamplification

STANDRAD VERSIONS:

Prelude™ One-Step PreAmp Master Mix

• Supplied as ready to use Master Mix

Prelude PreAmp Master Mix

Optimized reverse transcriptase, polymerase, and buffer in Prelude One-Step PreAmp MasterMix maximize RT-PCR efficiency for all targets. It ensures preamplification of >100 targets, from as little as 100 pg mRNA or 10 ng total RNA.

Main features:

• 2X PCR mix optimized for preamplification of multiple (>100) targets
• Ideal for a limited (10 pg–100 ng) input of cDNA or gDNA
• Suitable for work with FFPE tissue, biofluids, laser capture microdissection or exosomes
• Amplified products can then be used for qPCR, genotyping, or target enrichment analysis
• Ready-to-use—just add cDNA or gDNA samples and primers
• Single tube reaction format

STANDARD VERSIONS:

Prelude™ PreAmp Master Mix

Supplied as ready to use Master Mix