Retroviral Transduction

Retroviral transduction found its use in cancer and stem cell research. It allows transduction into dividing cells. However, retroviruses also incorporate their single stranded RNA into double stranded DNA of the host cell. Takara Bio offers wide portfolio of prodict covering whole workflow from packaging to retroviral titration.

1) RETROVIRAL PACKAGING

The Retro-X Universal Packaging System is a premium retroviral packaging system. Takara offers Retro-X System, Pantropic Retroviral Expression System and AmphoPack 293 Cell line.

Main features:

• GP2-293 packaging cell line what is very easy to transfect
• All four envelopes supplied on separate vectors including VSV-G, ecotropic, amphotropic and dualtropic
• Virions can be obtained in less than in 48 hours
• This system produces the highest titers

2) RETROVIRAL TRANSDUCTION TOOLS

Retro-X Concentrator
Retro-X Concentrator has simple mix and spin protocol without need of ultracentrifugation. Vector supernatants can be concentrated 10–100 fold, depending upon the volumes and envelope used.

Main features:

• No ultracentrifugation needed
• Simple mix-and-spin protocol
• 100X concentration
• 90% yield

Retro-X™ qRT-PCR Titration Kit
Retro-X qRT-PCR Titration Kit provides a simple and fast method for measuring retroviral titer and enables to confirm the success of the retroviral packaging reaction.

Main features:

• qRT-PCR based kit
• Perform consistent experiments, control copy number, and hence expression levels
• Works with all MMLV-based retroviral vectors (not MSCV)
• Results in approx. 4 hours

Retro-X Integration Site Analysis Kit
Retro-X Integration Site Analysis Kit enables to identify the exact site of integration of recombinant retroviral vectors using GenomeWalker technology. It is compatible with most commercially retrovirus vectors.

Ecotropic and Amphotropic Receptor Boosters
Are used to temporarily elevate the viral receptor proteins on the surface of the target cell. It allows to efficiently transduce human cells with ecotropic retrovirus or lentivirus that otherwise can only infect rodent cells.

3) RETROVIRAL VECTORS

Constitutive Vectors
Retroviral vectors transduce a wide range of hard to transfect cells and the complete system includes PT67 packaging cell line. Takara offers the Q-Series and L-Series of constitutive retroviral vectors.

Main features:

• Designed for high retroviral titers and high expression 
• Compatible with all Takara bio retrovila packaging cell lines

Fluorescent Retroviral Vectors

Enables the retroviral transduction for hight efficiency gene delivery to a broad range of cell types. The bright red and green fluorescent proteins can be used for reporter studies as well it is ideal for fusion studies.

Tet- Inducible Expression Systems
Enables the induction control and low basal expression for a broad range of dividing cells. TRE3G promoter is optimized for very tight transcriptional control and the kit is supplied with the Retro-X Universal packaging system.

MSCV Retroviral System
The MSCV (murine stem cell virus) Retroviral Expression System contains the vectors optimized for introducing and expressing target genes in pluripotent cell lines, including murine or human hematopoietic, embryonic stem and embryonal carcinoma cells.