RT-PCR Kits

High-quality reverse transcription reagents for PCR

1. PrimeScript based kits

This recombinant and modified MMLV reverse transcriptase is efficient preparation of cDNA up to 12 kb in length. PrimeScript is suitable for all applications where full-length DNA is required including cDNA library preparation or RT-qPCT.

Main Features:

Works on challenging templates (GC rich templates and templates with high levels of secondary structure)
Capable of extending up to 12 kb templates with minimal background
cDNA synthesis and extension through complex RNA structure at 42 °C
High specificity: reduced mispriming increases reverse transcription efficiency
High accuracy reverse transcription

ONE-STEP VERSIONS:

PrimeScript First Strand cDNA Synthesis Kit

Kit full-length cDNA synthesis primers included in the kit
Full kit with all reagents needed including Oligo dT Primer and Random 6-mers

PrimeScript™ One Step RT-PCR Kit Ver.2

One-step RT-PCR in a single tube
Includes PrimeScript 1 step Enzyme Mix (reverse transcriptase and TaKaRa Ex Taq HS) and 2X 1 step buffer (with dNTPs)
Two version: with or without gel loading dye

TWO-STEP VERSIONS:

PrimeScript RT-PCR Kit

For 2-step RT-PCR
Kit includes all reagents for reverse transcription and also Takara Ex Taq HS for cDNA amplification

PrimeScript™ High Fidelity RT-PCR Kit

Suitable for cDNA cloning studies with extremely high fidelity
Complete kit with all reagents needed including positive control

OTHER VERSIONS:

PrimeScript™ Double Strand cDNA Synthesis Kit

Kit designed for double stranded cDNA synthesis (Gubler-Hoffman method)
Resulting cDNA can be cloned to the vector and cDNA library can be generated
Complete kit with all reagents needed including controls

2. AMV Reverse Transcriptase XL based

AMV Reverse Transcriptase XL enables synthesis of full-length cDNA from single-stranded RNA, DNA, or RNA-DNA hybrid templates. It can be used over a temperature range of 37–55°C, but with optimal temperature between 41–44°C. It can be used for full-length cDNA synthesis from templates of up to 10 kb in length.

Main Features:

Synthesize long, full-length cDNA up to 11.7 kb
Requires a primer to initiate synthesis and Mg²⁺ or Mn²⁺ for activity
Get full-length cDNA from single-stranded RNA, DNA, or RNA-DNA hybrid templates
It has 5→3' ribonuclease H activity

ONE-STEP VERSIONS:

One-Step RNA PCR Kit (AMV)

Time saving one-step RT-PCR performed
Contains AMV-Optimized Taq DNA Polymerase for efficient cDNA amplification
Complete kit with all reagents needed including controls

TWO-STEP VERSIONS:

RNA PCR Kit (AMV) Version 3.0

Designed for single tube reverse transcription of RNA to cDNA
Allows also amplification of unknown 3'-termini using 3'-RACE
Kit contains all reagents for reverse transcription and cDNA amplification and also includes reagents for 3'-RACE

RNA LA PCR™ Kit (AMV) Ver. 1

Designed for generation of long first-strand cDNA products (up to 12 kb in length)
Protocol is performed in single tube
Kit includes Takara LA Taq for cDNA amplification
Kit contains all reagents for reverse transcription and cDNA amplification and also includes reagents for 3'-RACE

3. MMLV Reverse Transcriptase based

Recombinant MMLV RT is fully active RT that is free of exogenous RNases and other nucleases, so it prevents template RNA degradation. It allows synthesis of long, full-length cDNA of up to 11.7 kb in length.

ONE-STEP VERSIONS:

Titanium® One-Step RT-PCR Kit

For quantitative gene expression analysis from multiple samples
Easy to use single tube format
Starts even with 10 pg of total RNA
Provided as a complete RT-PCR kit (not including reagents for RNA purification)

TWO-STEP VERSIONS:

Advantage® RT-for-PCR Kit

Allows analysis of multiple genes from a single RNA source
First-strand cDNA synthesis from nanogram quantities of RNA
Kit contains all reagents for reverse transcription and cDNA amplification

4. BcaBEST DNA Polymerase based

BcaBEST DNA Polymerase contains both DNA polymerase and reverse transcriptase properties. Optimum reaction temperature of tis enzyme 65°C, thanks to this feature BcaBEST DNA polymerase is suitable for RT-PCR of long or difficult RNA templates. Maximum template length is 5 kb.

Main Features: