Tapestri Platform
Single-cell DNA Multi-omics platform for revelation of genetic diversity across cell populations
Droplet microfluidics platform allows access to DNA at the single-cell level. Two-step encapsulation workflow provides flexibility for customization. Released, barcoded, and amplified DNA is ready for NGS library preparation.
Main features:
- Targeted detecting of SNVs, CNVs and proteins simultaneously from the same cell
- Information about genotype and phenotype
- Single-cell DNA throughput up to 10,000 cells
- Detect rare subclones down to 0.1%
- Reveal true heterogeneity of cell population
- Clonal architecture or mutation co-occurrence analysis for treatment response, resistance and/or relapse monitoring
- Simple workflow and user-friendly bioinformatics software
- Catalogue, Published and Virtual Panels, customizable content
- Compatible with Illumina NGS platform
Tapestri workflow
1st encapsulation: Partitioning of cells into droplets along with a protease enzyme mix.
2nd encapsulation: Cell lysate combined with barcoded beads, primers, and reagents to amplify and tag each amplicon with a unique cell barcode for cell’s identity.
Next steps: Library preparation and sequencing on Illumina NGS platforms (MiSeq, NextSeq 550, HiSeq 2500, HiSeq 4000, NovaSeq 6000), data analyzes and visualization by Mission Bio Software.
