Tetracycline-inducible expression systems

1) Tet-One

Provide both of these components on a single vector. The Tet-On 3G transactivator is expressed in the forward direction from the human phosphoglycerate kinase 1 promoter, and the cloned gene of interest is expressed from the PTRE3GS promoter in the reverse orientation.

Main features:

• All-in-one vector design provides all the power of Tet-On 3G in a single vector, making inducible expression as easy as constitutive expression
• Low background and high maximum induced expression
• High sensitivity to doxycycline: Near maximum expression at 10 ng/ml doxycycline
• Inducible expression in a broad range of tissues, because the Tet-On 3G Transactivator is expressed from the human PGK promoter
• High-titer lentivirus packaging system provided with Lenti-X Tet-One Systems
• Retro-X System incudes our Retro-X Universal Packaging System
• The AAVpro system uses a helper-free packaging system and includes extraction reagents for efficient isolation of AAV particles
• Tightly controlled inducible expression in mammalian cells

2) Tet-On 3G

The Tet-On 3G Tetracycline-Inducible Gene Expression Systems are the 3rd generation of the most powerful, versatile, and widely cited inducible mammalian expression systems available. 

Main features:

• Tetracycline transactivator, Tet-On 3G, is expressed from an EF1-alpha promoter
• Very low background expression
• High induced expression

3) Bidirectional Tet-On 3G Inducible Expression Systems

The response vector supplied with these Tet-On 3G tetracycline-inducible expression systems contains a bidirectional version of our premium TRE3G promoter (PTRE3G-BI) that allows for simultaneous, equivalent and inducible expression of two transgenes.

Main features:

• Equivalent co-regulated and simultaneous expression of two genes
• Indirectly monitor expression of a target gene via co-induced expression of a bright green or red fluorescent protein
• Same tight control as all Tet-On 3G Systems
• Highly sensitive to doxycycline

4) Bicistronic IRES Tet-On 3G Inducible Expression Systems

The response vector supplied with these Tet-On 3G tetracycline-inducible expression systems express two transgenes from our premium TRE3G inducible promoter (PTRE3G).

Main features:

• Tightly controlled inducible expression of two genes of interest from the same bicistronic transcript
• Monitor induced expression of a single gene using mCherry or ZsGreen1 fluorescent proteins
• Two transgenes or one transgene and a bright fluorescent protein expressed from a single IRES bicistronic transcript

5) Lentiviral Tet-On 3G Inducible Expression Systems

The Lenti-X Tet-On 3G Inducible Expression Systems combine the broad cellular tropism of lentiviral gene transfer technology with the power and versatility of our state-of-the-art tetracycline-regulated gene expression system.

Main features:

• Low basal expression, high maximal expression, highly controllable
• Lenti-X Packaging Single Shots (VSV-G), included with each system, generates high titers with a very high safety profile
• TRE3G promoter provides very tight control of transcription

6) Tet-On 3G Lentiviral Particles—Purified and Concentrated

Ready-to-use, purified, high titer lentiviral particles that deliver the Tet-On 3G transactivator to target cells.

Main features:

• Premium quality ready to use Tet-On 3G lentivirus
• Very high titer; functional titer of >1 x 109 transduction units per ml (TU/ml)
• Functional titer provided, not a just a particle count
• High purity - transduce target cells without cytotoxicity

7) Retroviral Tet-On 3G Inducible Expression Systems

The Retro-X Tet-On 3G Inducible Expression System combines the efficiency of retroviral gene transfer with the power of our premium tetracycline-regulated gene expression technology.

Main features:

• Outstanding induction control and extremely low basal expression for a broad range of dividing cell types
• TRE3G promoter is optimized for very tight transcriptional control
• Supplied with our best-performing retroviral packaging system (Retro-X Universal)

8) Adeno-X Expression System 3 (Tet-On 3G Inducible)

Clontech’s Adeno-X Adenoviral System 3 (Tet-On 3G Inducible) combines the tightest and most sensitive control of gene expression with the most advanced commercially available adenoviral vector system.

Main features:

• Very tight control of gene expression
• Simple-to-use, all-in-one tetracycline inducible system
• The most advanced adenoviral gene delivery technology
• Easiest adenoviral system to use; cloning is even simpler than standard plasmid cloning

9) Tet-On 3G Cell Lines for Tetracycline-Inducible Expression

Tet-On 3G cell lines greatly simplify the task of setting up a tetracycline-inducible gene expression system that is activated by adding doxycycline (Dox) to the culture medium.

Main features:

• Cell lines with high inducibility
• Rapidly create an inducible expression system for your gene of interest.
• Premade Tet-responsive cell lines reduce development time/effort by half