Two-Step RT-qPCR

1. DYE-BASED DETECTION

CellAmp Direct TB Green RT-qPCR Kit

Tool designed for simple two-step RT-qPCR reaction. The formulation does not require RNA extraction and reverse transcription can be performed directly from animal cell samples (e.g., adherent cells, suspension cells, primary cells, various stem cells, and iPS cells). Also, effective elimination of genomic DNA is possible.

Main features:

• Works with crude samples
• Reverse transcription reactions and gene expression analysis in 1.5 hours
• Lysates can be stored for a long time (-20°C per 6 months at least)
• Resistant to PCR inhibitors
• Suitable for samples with high GC pair content

STANDARD VERSIONS:

CellAmp™ Direct Lysis and RT set

• Includes lysis and washing buffers and RT reagents supplied in separate tubes

CellAmp™ Direct TB Green® RT-qPCR Kit

• All solutions for RT and qPCR are supplied in the kit, including PrimeSript enzyme mix and TB Green Fast qPCR Mix and ROX reference dye in separate tubes

2. PROBE-BASED DETECTION

CellAmp Direct Probe RT-qPCR Kit

Tool designed for simple two-step RT-qPCR reaction. The formulation does not require RNA extraction and reverse transcription can be performed directly from animal cell samples (e.g., adherent cells, suspension cells, primary cells, various stem cells, and iPS cells). Also, effective elimination of genomic DNA is possible.

Main features:

• Works with crude samples
• Reverse transcription reactions and gene expression analysis in 1.5 hours
• Lysates can be stored for a long time (-20°C per 6 months at least)
• Resistant to PCR inhibitors
• Suitable for samples with high GC pair content

STANDARD VERSIONS:

CellAmp™ Direct Lysis and RT set

• Includes lysis and washing buffers and RT reagents supplied in separate tubes

CellAmp™ Direct Probe RT-qPCR Kit

• All solutions for RT and qPCR are supplied in the kit, including PrimeSript enzyme mix and probe qPCR mixes and ROX reference dye in separate tubes