Ultra-Low-Input RNA-Seq

Robust and sensitive enough NGS library preparation methods for reproducibly capture RNA molecules present in minute quantities.

 Main features:

• cDNA synthesis from 1 - 1,000 cells, or 10 pg –10 ng high-quality (RIN>8) total RNA
• Kits use oligo(dT) priming
• Rely on proprietary SMART (Switching Mechanism at 5’ end of RNA Template) technology
• SMART technology ensures full-length unbiased mRNA coverage
• Powerful approach for resolving biologically relevant differences between individual cells
• Analysis of rare or precious samples (stem cells, circulating tumor cells and tissue biopsies)
• Improved chemistry relative to previous versions and locked nucleic acids (LNA)-enhanced oligos
• Accurate representation of GC-rich transcripts
• Compatible with Illumina or Ion Torrent sequencing platforms

AVAILABLE KITS:

SMART-Seq® mRNA HT LP

• Input range: 1–100 cells or 10 pg–1 ng of total RNA
• Easy to automate workflow
• Capture full-length transcript coverage, a low percentage of rRNA reads, and a broad representation of GC-rich transcripts
• Designed for cDNA synthesis and library preparation
• UDIs sold separately

SMART-Seq® mRNA LP

• Input range: 10 pg–100 ng total RNA (RIN ≥ 8), or 1–1,000 cells
• Designed for cDNA synthesis and library preparation
• Identify the highest number of genes; detect gene fusions, isoforms, SNPs
• UDIs sold separately