High Yield PCR Polymerases

TaKaRa Bio

Ex Taq DNA Polymerase

• Combines Taq polymerase with the proofreading activity of an efficient 3' to 5' exonuclease
• < 100 bp to 20 kb for genomic DNA targets
• Less optimization

 

	Ex Taq	Ex Taq HS	Premix Ex Taq
Applications	Routine PCR, Long PCR, TA Cloning	Routine PCR, Long PCR, TA Cloning, Specificity	Routine PCR, Long PCR, TA Cloning
Features	DNA proofreading activity	Hot Start, Antibody mediated, DNA proofreading activity	Optimised Master mix, DNA proofreading activity
Additional coments	Available with Mg2+ free buffer	Available as premix	-

 

 

Titanium Taq

• More robust and allows amplification of highly complex DNA mixes
• Contains carefully engineered amino acid substitutions- increases its solubility,
• The most sensitive PCR polymerase available
• Lacks 5'-exonuclease activity
• Tolerates wide range of Mg²+ concentrations
• Targets of up to 2 kb from highly complex templates

 

	Titanium Taq	Tutanium DNA Amplification Kit
Application	Genotyping, Whole genome PCR, Multiplex PCR, Primer extension, Hot start PCR, Preparative PCR, Rare templetes	Genotyping, Cytogenetics, Genome-Wide Human SNP Assay
Additional comments	Available in EcoDry format	Optimized for Affymetrix CytoScan HD Ar-ray, CytoScan 750K* Array, Genome-Wide Human SNP Array 6.0

 

 

Advantage 2 Polymerase

• Optimized blend of proofreading DNA polymerase and Titanium Taq DNA polymerase
• Provides a proven balance of high yield and high fidelity
• For cDNA amplification and library construction that requires minimal optimization
• Provides nearly error-free amplification
• Target DNA up to 5 kb
• Available in a lyophilized format as High Fidelity PCR EcoDry Premix

 

	Advantage 2 Polymerase Mix	Advantage 2 PCR Kit
Application	Long and accurate PCR Preparative PCR RACE PCR cDNA synthesis/amplification cDNA subtraction Hot start PCR	Long and accurate PCR Preparative PCR RACE PCR cDNA synthesis/amplification cDNA subtraction Hot start PCR
Features	Hot start Proofreading High Yield High Fidelity	Convenience Hot start High Yield High Fidelity

 

 

EmeraldAmp

• High yield: at least 10X more end product than conventional PCR
• Use in TA cloning or direct sequencing without purification, Long PCR, High yield PCR
• Perform restriction digests directly in the PCR buffer
• Dye added to buffer
• 10X more end product
• Targets up to 10 kb in length

 

	EmeraldAmp Max PCR Master Mix	EmeraldAmp Max HS PCR Master Mix
Applications	Specificity– Optimised buffer High Yield Long PCR	Long PCR High yield PCR
Features	Specificity– Optimised buffer High Yield Long PCR GC Rich 10X more end product Targets up to 10 kb in length	Hot Start Specificity– Optimised buffer High Yield Long PCR GC Rich 10X more end product Targets up to 10 kb in length

 

SuperPlex Premix

• 2X, hot-start PCR master mix ideal for multiplex PCR as well as high-yield singleplex PCR
• Formulated with high-yield Titanium Taq DNA polymerase
• Provides unbiased amplification of different targets and high amplicon yield
• The premix formulation minimizes pipetting steps and streamlines workflows
• Just add primers, sample, and water
• Two size of premix for 200 Rnxs and 1000 Rxns

 

Applications of multiplex PCR

• Pathogen detection/identification
• High-throughput SNP genotyping
• Mutation analysis
• Gene deletion analysis
• Template quantification

 

 

Ex Premier DNA Polymerase

• All around great performance
• Highest success rate of all Takara Bio polymerases
• High fidelity (has 3′ -> 5′ exonuclease activity)
• Suitable for long amplicons, up to 30 kb (human genomic DNA) and AT/GT-rich amplicons
• Faster amplification—amplifies targets at 30 sec/kb
• Works with crude samples:
  • Animal tissue sample (e.g., mouse tail extract)
  • Plant tissue sample (e.g., leaf extract)
  • Blood sample (e.g., serum, plasma and whole blood)
• Can be used in In Fusion cloning, NGS, Sanger, etc...
• Gel-loading dye format also available
• Ease-of-use 2X premix - add only sample and primers

	Ex Premier DNA Polymerase	Ex Premier DNA Polymerase Dye plus
Applications	Long PCR High yield PCR	Long PCR High yield PCR
Features	Works with crude samples High Yield Long PCR GC Rich Targets up to 30 kb in length	Hot Start High Yield Long PCR GC Rich Dye included  Targets up to 30 kb in length

Documents and resources

For more information visit producer's website.