Baculovirus Expression Systems

Baculovirus expression systems are well known for its capability of expression of large amounts of recombinant proteins in insect cells. System uses the baculovirus Autographa californica nuclear polyhedrosis virus (AcMNPV) to produce target proteins. To force recombination between virus and transfer vector, BacPAK6 Viral DNA is being cotransfected with the target gene. After purification and phenotype verification newly isolated recombinant virus can be amplified and used for cell infection.

Main features:

• Yields up to 1 g of protein per liter of culture
• Recombinant efficiency >90%
• Proper protein folding
• Expression of biologically active proteins
• Eukaryotic posttranslational modification

Insect cell line for Baculovirus expression

Cells designed to be used together with BacPAK system. The IPLB-Sf21 cell line is derived from pupal ovaries of the fall armyworm, Spodoptera frugiperda. Exponentially growing IPLB-Sf21 cells are concentrated by centrifugation and frozen in insect cell complete medium containing 10% dimethysulfoxide (DMSO).

Baculovirus titration kits

Based on detection of expression of either the baculoviral gp64 protein or p35 protein, BacPAK ttitration kits provide rapid, simple, and effective method for baculovirus quantification in cell culture supernatants. Available are version with low, medium and high sensitivity.

Main features:

• Shorten baculovirus expression experiments by up to six days
• Eliminate troublesome plaque assays and end-point dilution assays
• Use with any AcMNPV-based baculovirus expression systems
• Harvest, titer, and infect in a single day

AVAILABLE VERSIONS:

BacPAK Baculovirus GoStix Plus

• Method: Lateral flow assay
• Based on: gp64 expression
• Assay time: 10 min
• Sensitivity: Low

BacPAK p35 ELISA Kit (Single Wash)

• Method: ELISA
• Based on: gp35 expression
• Assay time: 20 hrs
• Sensitivity: Medium

BacPAK Baculovirus Rapid Titer Kit

• Method: Immunological staining
• Based on: gp64 expression
• Assay time: 2 days
• Sensitivity: Medium

BacPAK qPCR Titration Kit

• Method: qPCR
• Based on: viral genome content
• Assay time: 4 hrs
• Sensitivity: High