Fluorescent Proteins and Reporters

1) FLUORESCENT PROTEINS

Portfolio of 20 bright and photostable fluorescent proteins for every possible gene function and expression study.

AVAILABLE FLUORESCENT PROTEINS:

• AmCyan1 Fluorescent Protein (ideal for multicolor labeling)
• AsRed2 Fluorescent Protein 
• AcGFP1 Fluorescent Protein (AcGFP1 and EGFP) 
• mBanana Fluorescent Protein (Bright yellow fluorescent protein) 
• mCherry Fluorescent Protein (mCherry and PAmCherry) 
• Dendra2 Fluorescent Protein (Irreversible switching from green to red is activated by UV-violet or blue light)
• DsRed2 Fluorescent Protein (High solubility and low aggregation)
• DsRed-Express Fluorescent Protein 
• DsRed-Monomer Fluorescent Protein (Monomeric structure)
• E2-Crimson Fluorescent Protein (Far-red fluorescent protein, E2-Crimson) 
• GFP & GFPuv Fluorescent Proteins 
• HcRed1 Fluorescent Protein (Far-red fluorescent protein, HcRed1) 
• mOrange Fluorescent Protein (mOrange2 and mOrange)
• PAmCherry Fluorescent Protein (Non-fluorescent until activated by a short exposure to 350–400 nm light) 
• mPlum Fluorescent Protein (Far-red fluorescent protein) 
• mRaspberry Fluorescent Protein (Far-red fluorescent protein) 
• mStrawberry Fluorescent Protein
• tdTomato Fluorescent Protein
• Timer Fluorescent Protein (DsRed1-C Sequencing Primer to sequence wild-type DsRed1 C-terminal gene fusions) 
• ZsGreen1 Fluorescent Protein (Exceptional brightness: 2.5 times brighter than EGFP
• ZsYellow1 Fluorescent Protein (Bright yellow fluorescent protein)

2) FLUORESCENT PROTEIN ANTIBODIES

Antibodies prepared for various applications such as Western blot, immunoprecipitation, immunocytochemistry or immunohistochemistry. Takara Bio offers wide variety of antibodies for different fluorescent protiens.

AVAILABLE ANTIBODIES:

• Cyan Fluorescent Protein Antibodies 
• Far Red Fluorescent Protein Antibodies (Specific for Far Red Fluorescent Proteins - mPlum, mRaspberry, E2-Crimson, HcRed1)
• Green Fluorescent Protein (GFP) Antibodies
• Orange Fluorescent Protein Antibodies (mOrange2 and mOrange antibodies)
• Red Fluorescent Protein Antibodies (mCherry, tdTomato, DsRed-Express, DsRed-Monomer)
• Yellow Fluorescent Protein Antibodies

3) FLUORECENT PROTEIN VECTORS

Bacterial Expression Vectors

Vectors have a lac promoter and are intended to be used as expression vectors in bacterial systems. Vectores can also be used as a source of different fluorescent protein coding sequences (genes), which can be amplified by PCR.

EF-1 Alpha Promoter Vectors

• Human elongation factor-1 alpha (EF-1 alpha) is a constitutive promoter of human origin that can be used to drive ectopic gene expression in various in vitro and in vivo contexts.
• Living Colors fluorescent protein fusion vectors
• IRES vectors for coexpression of 2 genes
• Vectors with highly immunoreactive Myc or HA tags
• Can be used to confirm Matchmaker interactions in mammalian cells

Fucci Cell Cycle Vectors

• Cell cycle reporter vectors allow monitor cell cycle progression in living cells, in real-time, without fixation. They even let you visualize cell shape.
• Retroviral vectors to track cell cycle phase in living cells
• Differentiate G1 phase from S/G2/M based on color
• See the complete morphology of the cell during simultaneous monitoring of cell cycle phase

Fusion protein products

• Cyan Fluorescent Protein- AmCyan1 Fluorescent Protein
• Far Red Fluorescent Proteins
• Green Fluorescent Protein (GFP) Fusions- AcGFP1 & ZsGreen1
• mOrange Fluorescent Protein
• Photoswitchable Fluorescent Protein-Dendra2
• Red Fluorescent Proteins
• Yellow Fluorescent Proteins-mBanana and ZsYellow1

MicroRNA Expression Vectors

• These vectors provide strong miRNA expression and allow you to verify and track that expression with a fluorescent protein marker.
• Strong coexpression of miRNA and a bright fluorescent protein
• Clone your miRNA into the 3'UTR of mCherry or ZsGreen1
• Simultaneously verify miRNA expression and transfection efficiency via fluorescent microscopy

Photoactivatable-mCherry Fluorescent Protein

• Living Colors PAmCherry is a photoactivatable fluorescent protein (PAFP) derived from the red fluorescent protein mCherry. PAmCherry is non-fluorescent until it is exposed to 350–400 nm light.
• Non-fluorescent until activated by a short exposure to 350–400 nm light
• Excitation maximum: 564 nm
• Emission maximum: 595 nm

Photoconvertible Fluorescent Proteins-Dendra2

• Dendra2 is a photoswitchable (green to red), monomeric fluorescent protein derived from octocoral Dendronephthya sp.
• Irreversible switching from green to red is activated by UV-violet or blue light
• Switched (red) form is highly photostable
• Matures at a wide range of temperatures

Promoter reporters

• Promoterless (Lowest Background)
• On-demand reporter vectors are the next generation of promoter reporters. With these reporters systems, you can compensate effectively for reporter background without compromising your assay’s signal intensity.
• Promoterless (Traditional)
• Monitor transcription from different promoters or promoter/enhancer combinations inserted into the MCS located upstream of the Living Colors fluorescent protein coding sequence.

Recombinant Fluorescent Proteins—GFP, DsRed

• These Living Colors recombinant proteins are purified from E. coli. Each recombinant variant has absorption and emission spectra identical to those for the expressed proteins.
• Purified, recombinant proteins from E. coli
• Protein retains the absorption and emission spectra identical to expressed proteins
• Retains fluorescence capability

Organelle and Subcellular Labeling

• Visualize subcellular structures directly and noninvasively by fluorescence microscopy.
• Localize fluorescence to specific organelles or structures in living cells
• Visualize biological processes as they occur
• Dual- or triple-label cells with different fluorescent proteins

Transfection Control

• The Living Colors Constitutive Fluorescent Protein Expression Vectors can be used as transfection markers and to label living cells.
• Bright fluorescent proteins for labeling
• Choice of CMV or EF1-a promoters

4) CHEMILUMINISCENT REPORTERS

Colorimetric assay

• The Beta-Galactosidase Staining Kit
• Complete system for colorimetric detection of beta-galactosidase expression in mammalian cells

Chemiluminescent assay

•  The Luminescent Beta-Galactosidase Reporter System 3
• Provide quantitative measurement of beta-gal activity using a chemiluminescent substrate

pBeta-Gal-Basic

• Eukaryotic promoter and enhancer sequences
• Serve as a negative control or to clone promoters for characterization

pBeta-Gal-Control

• Contains the SV40 promoter and enhancer sequences
• Can be used as a positive control or as a reference for comparing the activities of promoters and enhancers

pCMV-LacZ

• Constitutive mammalian reporter vector
• Can be used as a reference or control plasmid
• Expresses extremely high levels of beta-galactosidase from the human cytomegalovirus immediate early promoter (PCMV IE) 

In Vivo Luciferase Imaging Kit

• Provides a bioluminescent assay
• Simplifies the analysis of promoter function in live cells or live animals
• Fast and easy—detect promoter activity in live cells and live animals
• For live-cell and live-animal imaging
• Allows repeated assaying over time

Ready-To-Glow Secreted Luciferase Assay

• Based on the secreted Metridia luciferase reporter.
• Combines the advantages of a live-cell assay with the sensitivity of an enzyme-based system
• Secreted luciferase assay protocol eliminates cell lysis
• 2–4 fold higher signal than firefly or Renilla luciferase
• Single transfection allows multiple time points from same cell

ProLabel Chemiluminescent Assay

• Provide sensitive, chemiluminescence-based assays
• For detecting and measuring the expression of any recombinant protein of interest
• Western blot kit alternative: highly sensitive, easy to use
• A quantitative enzymatic assay that’s simple and fast
• High-throughput compatible

Secreted Alkaline Phosphatase Assay

• The Great EscAPe SEAP 2.0 System uses a secreted alkaline phosphatase assay
• For analysis cis-acting DNA sequences and trans-acting factors
• One step assay, for monitoring promoter activity
• 10-fold higher sensitivity then firefly luciferase
• No-cell-lysis protocol