Phospho and Glycoproteins Enrichment

1) Glycoprotein Enrichment

Glycoprotein Enrichment Resin is used for the enrichment of glycosylated proteins from serum, significantly reducing sample complexity in proteomics studies.

• Can be used with gravity flow or FLPC columns
• Shows increased specific binding of glycoproteins and reduced nonspecific binding
• For low and high-abundance serum glycoproteins
• Fast protocol (90 minutes)
• Detection kit provides rapid sensitive, and specific antibody-INDEPENDENT Western detection
• The Glycoprotein Western Detection Kit is also avaible as an accessory product

2) Phosphopeptide Enrichment

Phosphopeptide Enrichment Spin Columns and Buffer Kit enhance detection of phosphorylated peptides that would otherwise be undetectable. The straightforward protocol enriches your protein digests prior to detection by mass spectroscopy or HPLC.

• Efficient, specific enrichment for all types of phosphopeptides (phosphotyrosine, phosphoserine, or phosphothreonine)
• Spin columns contain a unique immobilized metal affinity chromatography resin that binds phosphopeptides
• Columns have the capacity to bind up to 250 µg of phosphopeptide and can accommodate up to an 850 µl sample volume.

Phosphoprotein Enrichment Kit provides an effective affinity-based procedure for isolating phosphorylated proteins from mammalian cells and tissues.

• Enrichment of both cytosolic and membrane-bound phosphoproteins
• The average cell-to-sample purification time is less than 2 hours
• A single buffer-Extraction/Loading Buffer-is used for both the protein extraction and affinity column steps
• The procedure is nondenaturing, so phosphoproteins remain folded throughout the process, even during the extraction and elution steps.

Magnetic Phospho Enrichment Kit TALON PMAC combines the phosphospecificity of TALON-based Phosphoprotein Enrichment Kit with the convenience of magnetic bead separation.

• A simple, metal affinity-based procedure for microscale phosphoprotein purification
• Quick and easy enrichment from any cell or tissue sample
• Nondenaturing protocol maintains protein conformation and solubility
• Eluted samples ideal for small volume applications