Takara Bio
Single-Cell RNA-Seq
Best-in-class tools for sequencing library preparation from single cells and ultra-low-inputs of RNA
Robust and sensitive enough NGS library preparation methods for reproducibly capture RNA molecules present in minute quantities. Takara bio kits generate high-quality, full-length cDNA directly from different single cell types.
Main features:
- Input material are single cells
- Good results with low RNA content cells like peripheral blood mononuclear cells
- cDNA is compatible with lllumina platform-specific library preparation kits
- Easy, plate-based workflow - direct input of single cells isolated by FACS or other methods
- Highly scalable workflow - high-throughput capabilities and automation-friendly
AVAILABLE KITS:
SMART-Seq® Total RNA Single Cell (ZapR® Mammalian)
- Input range: 1–1,000 intact mammalian cells or 10 pg–10 ng (RIN >4)
- Libraries prepared in ~6.5 hours
- Includes 8 nucleotide (nt) unique molecular identifier (UMI)
- Mammalian rRNA depletion chemistry is part of the kit (ZapR Mammalian)
SMART-Seq® mRNA Single Cell LP
- Input range: 1 cell
- Robust, full-length chemistry
- Designed for cDNA synthesis and library preparation
- Works well even with cells with very low RNA content and nuclei
- Automation friendly
- UDIs sold separately
Dokumentumok és források
For more information about SMART-seq Single Cell Kit visit producer’s website.
