Takara Bio
SMARTer ICELL8 cx Single-Cell Analysis System
Single-cell analysis system for isolation and selection of cells of interest
Advanced automation platform which integrates cells dispensing and imaging to achieve the isolation of single cells. The ICELL8 cx utilize 5,184-nanowell chips and large-bore nozzle dispenser to accommodate large cells without compromising their viability or transcriptome profile.
Main features:
- Up to 8 samples on each chip
- Usable cell size for unbiased cell isolation: 5–100 µm
- Isolation of 1,000–1,800 single cells in a 5,184-nanowell chip
- Rapid dispensing (15 min) and imaging (12 min)
- Many validated cell types (e.g. nuclei, cardiomyocytes, B lymphocytes, neurons, spheroids, etc.)
- Multiple imaging channels (red, blue, green)
- Choice of specific cells (cells of interest) to process using advanced imaging software
- Reagents optimized to enhance cell separation and viability
- Blank chips and preprinted chips for specific applications
- Flexibility to use pre-validated SMARTer NGS chemistries or developed custom applications
SMARTer ICELL8 cx Single-Cell System includes:
- Thermal Cycler
- MultiSample NanoDispenser (MSND)
- Imaging station
- CellSelect Software
MultiSample NanoDispenser (MSND)
- Delivers nanoliter volumes of cells and reagents
- Rapid dispensing to ensure cell viability
- Temperature and humidity control
Imaging Station
- Rapid image capture of individual wells on the chip
- Fluorescence detection of cells of interest
- Direct interface with CellSelect software
CellSelect Software
- Interrogates images of each well
- Determines the number of cells present and viability
- Identifies and recommends wells with single cells for downstream applications
Applications
- SMART-Seq full-length transcriptome analysis
- Differential expression by 3' end counting
- TCR profiling and 5′ differential expression
- ATAC-seq
- Custom applications
Dokumentumok és források
For more information visit producer’s website.
