Human iPS Cell Editing

TaKaRa Bio

The CRISPR/Cas9 became the powerful tool for gene editing because of its high targeting specificity, editing efficiency and simple use. Once the Cas9/sgRNA complex is delivered into the cells, the single cells must be isolated and expanded into clonal cell lines in order to isolate and screen for the genotype of interest.

Traditionally, the establishment of a clonal population from edited hiPS cells grown and passaged as colonies is inefficient, challenging, and time consuming; often, it results in cell death or premature differentiation. However, the cell culture component of Cellartis gene editing and single-cell cloning systems contains a defined culture system (composed of basal medium, coating, and additives) for efficient single-cell cloning and expansion of edited hiPSC clones.

 

Cellartis iPSC Single-Cell Cloning DEF-CS Culture Media Kit

• Complete system for efficient expansion
• Scales up manufacturing of hiPSCs in a feeder free and defined environment

 

Cellartis iPSC rCas9 Electroporation and Single-Cell Cloning System

• Complete system that allows efficient gene editing of hiPSCs via electroporation followed by single cell cloning and expansion into 48-well plates
• This system provides recombinant Cas9 as well as all the necessary reagents to produce high yields of sgRNAs for electroporation into hiPSCs

 

Cellartis iPSC CRISPR/Cas9 Gesicle and Single-Cell Cloning System

• Complete system that allows efficient gene editing of hiPSCs via gesicle delivery of Cas9/sgRNA complexes followed by single cell cloning and expansion into 48-well plates
• Gesicles are a non-toxic and efficient alternative to electroporation that do not rely on costly equipment or consumables

 

Documents and resources

For more information visit producer's website.