In-Fusion Cloning Kits

TaKaRa Bio

• Kits need ligation and are absolutely seamless
• Final construct have no extra base pairs left over
• The reaction last 15 minutes for single or multiple fragments
• Insert cloning yields are over 90 %
• Over 95% efficiency is demonstrated with a broad range of fragment sizes from 0,5-15 kb
• The large inserts of vectors are directly clone into the expression plasmid

 

You can choose from available kits

In-Fusion Snap Assembly (liquid form)

In-Fusion Snap Assembly EcoDry (lyophilized formula)

• Pre-aliquoted, lyophilized components that minimize handling errors
• Components can be stored at room temperature eliminating freeze-thaw cycles

In-Fusion Snap Assembly bundles

In-Fusion Snap Assembly for high-throughput workflows

In-Fusion HD Cloning bundles

• TaKaRa is in the process of discontinuing In-Fusion HD Cloning kits and strongly recommend ordering the latest In-Fusion product, In-Fusion Snap Assembly

Cloning Enhancer

 

Applications

• PCR Cloning
• HTP Cloning
• Directional cDNA cloning
• Mutation generation
• Multiple fragment cloning
• Adding adaptors, linkers, protein tags
• Cloning using modular parts
• High-throughput cloning
• Gene synthesis
• Gene design
• Site-directed mutagenesis
• Domain swapping
• Domain modification

 

Documents and resources

For more information visit producer's website.