naica® system for Crystal Digital PCR™

Stilla Technologies

Components of naica® system

• Shappire chips for 4 samples and 25 µl of PCR mix per reaction
• Ruby chips for 16 samples and 5 µl of PCR mix per reaction
• Geode (sample partitioning and thermal cycling)
• Prism (fluorescence reader)
• Crystal Miner software

 

Easy to use digital PCR

Fast time-to-result (under 4 hours) with less than 20 mins of hands-on time

Crystals of 10.000 to 30.000 droplets are created from each sample using patented microfluidic technology

PCR amplification is performed immediately after crystal generation

Crystals are imaged to reveal the droplets that contain amplified targets in multiple channels

3-color or 6-color detection capability and multiplexing:

• Prism3: Blue, Green and Red channel (for e.g. FAM, VIC/HEX, Cy5 fluorophores and their alternatives)
• Prism6: Blue, Teal, Green, Yellow, Red and Infrared channel (for e.g. FAM, Yakima Yellow, Atto550/Cy3, ROX, Cy5, Atto700/Cy5.5 fluorophores and their alternatives)

Software automatically counts the number of positive droplets in each population and converts it into absolute concentration of targeted DNA sequences in copies/µl.

 

Type of analysis:

• Absolute quantification (DNA/ RNA)
• Multiplex analyses
• Copy number variation
• Rare event detection
• Liquid biopsy
• Gene expression
• NGS library calibration/ NGS results validation sequences

 

The naica® system workflow

 

Reagents:

• ready-to-use
• two-component solution (buffer A and B)
• available in 5x and 10x concentration
• 10x concentration allows increased sensitivity for low concentration samples and rare targets 
• excluding DNA primers, probes/intercalating dye and DNA template

 

1) naica® multiplex PCR MIX 

• specially formulated for optimal TaqMan fluorescent probe-based Crystal Digital PCR™
• high multiplexing capacity
• contains an internal reference dye for partition detection and identification in the BLUE channel

 

2) naica® PCR MIX 

• optimised for EvaGreen fluorescent intercalating dye-based Crystal Digital PCR™
• Alexa Fluor 647 might to be added as reference dye to increase the basal fluorescence of droplets

 

Kits available:

	Concentration	Volume	Number of reactions Sapphire	Number of reactions Ruby
naica® multiplex PCR MIX	5x 5x 10x 10x	0.75mL 1.50mL 0.375mL 0.750mL	150 300 150 300	750 1500 750 1500
naica® PCR MIX	5x 10x	1.50mL 0.75mL	300 300	1500 1500

 

Documents and resources

For more information about the naica® system for Crystal Digital PCR™ visit producer's website.

Brochure naica® system for Crystal Digital PCR™