One-Step RT-qPCR

TaKaRa Bio

TB Green detection

1) One-Step TB Green PrimeScript RT-PCR Kit

• One-Step RT-PCR lowers pipeting and contamination risks
• Uses PrimeScript RTase, a robust reverse transcriptase
• Includes Ex Taq HS, a high efficiency hot start PCR enzyme to detect small amount of RNA
• Optimized for One-Step RT-qPCR with TB Green detection

 

2) One-Step TB Green PrimeScript RT-PCR Kit II

• Extremely high specificity : optimized buffer and anti-Taq antibody mediated  HS polymerase
• Easy and efficient : premixed components lower pipeting and contamination risks
• Sensitive: accurate quantification of any RNA including RNA viruses or small amounts of RNA
• Includes Ex Taq HS, a high efficiency hot start PCR enzyme

 

 

Probe detection

1) One-Step PrimeScript RT-PCR Kit

• Easy: One-Step RT-PCR lowers pipeting and contamination risks
• Includes Ex Taq HS, a high efficiency hot start PCR enzyme
• Sensitive: accurate quantification of any RNA with TaqMan probe detection
• Robust and efficient
• PrimeScript (Rnase H Minus MMLV-based) RTase ensures efficient transcription from any RNA tempalte even with high secondary structure

 

2) 5X One Step PrimeScript III RT-qPCR Mix

• Easy to use: One-step RT-qPCR, probe based
• Single tube protocol
• Includes specific TaKaRa Taq HS in an optimized reaction buffer and new heat- and inhibitor-resistant reverse transcriptase, PrimeScript III RTase
• cDNA synthesis from RNA with a more complex secondary structure
• Improving Limit of Detection (LoD) by allowing more space for sample in reaction mix
• ROX premix option available
• Automation friendly

 

 

Documents and resources

For more information visit producer's website.