Stem Cells Overview

TaKaRa Bio

Stem cells are still fascinating cells for both scientists and public and still there is so much to be discovered about them. TaKaRa Bio offers the whole portfolio of products may be used in research of the stem cells.

 

Generation of the Stem cells

The first step is the reprogramming of the cell and you can choose from the offer of reagents for both viral and transfection based delivery of reprogramming factors.

• XFect Transfection Reagent
  • Biodegradable transfection polymer
  • Used to transfect the stem cells with high efficiency and very low cytotoxicity
• Lentiviral particles
  • High-titer, high-purity, ready to use 
  • Deliver reprogramming factors
  • Both constitutive and inducible expression systems are available

 

Stem Cell Culture

Culture the stem cells is challenging, because it is necessary to ensure stemness, maintain genetic stability and prevent the spontaneous differentiation. TaKaRa Bio offers the media and supplements evolved specifically for culturing the stem cells.

 

Stem Cell Editing

Stem Cell Modification by CRISPR/Cas9 system for the gene editing can be used to modify the stem cells.

 

Stem Cell Differentiation

Needs optimized culture methods and reagents to ensure a reproducible outcomes. You can choose from ready stem cells.

• NDiff 227
  • Defined, serum-free medium
  • Supplemented with N2 and B-27 for neural differentiation of mouse embryonic stem cells
• RHB-A
  • Defined, serum-free medium
  • Differentiates mouse and human neural stem cells into functional neurons
  • Differentiates mouse ES cells into neuronal precursors
• Cellartis Enhanced hiPS-HEP cells
  • Highly pure human pluripotent cell lines of hepatocytes
  • Used in drug metabolism studies or various toxicity testing
• Cellartis Cardiomyocytes
  • Cardiomyocytes derived from human iPS cells 
  • Used for cardiotoxicity testing

 

Stem Cell Characterization

Requires variety of antibodies for characterizing pluripotency, differentiation, identifying and sorting of the human stem cells.

 

Documents and resources

For more information visit producer's website.